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Effects of nanoparticles in cells infected by Toxoplasma gondii

机译：纳米粒对弓形虫感染细胞的影响

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Core-shell model drug carriers on 2 nanoscale size levels have been applied in cell culture studies, focused on Toxoplasmosis therapy. In synthesis, a seed of Rhodamin B-labelled polystyrene latex particles was coated by polybutyl cyanoacrylate under physical inclusion of 2 different, new drugs against Toxoplasmosis. Drug-loaded as well as drug-free core-shell model drug carriers were added to a cell culture of human macrophages, infected by Toxoplasma gondii, following an infection plan. Drug release from the carriers had been studied before by enzymatic degradation by means of pork liver esterase. Particle size decrease by degradation was investigated in a UV/VIS spectrometer via transmission measurements. Drug release profiles were obtained by HPLC studies. The dynamics in the population of infected human macrophages, Toxoplasma gondii as well as model drug carrier numbers were registered by a FACS (fluorescence-activated cell sorter). As main result, the drug-free references in the 2 series of core-shell model drug carriers achieved ca.85% of the observed maximum in Toxoplasmosis therapy efficiency. These data were correlated with an immune stimulant effect on the side of the human macrophages, caused by the cell uptake of colloidal substrate, foreign to the body.
机译：2纳米大小水平的核壳模型药物载体已用于细胞培养研究，重点是弓形虫病治疗。在合成过程中，在物理包含2种不同的抗弓形虫病新药的条件下，用氰基丙烯酸丁酯包被了若丹明B标记的聚苯乙烯胶乳颗粒的种子。按照感染计划，将载有药物的药物以及不含药物的核壳模型药物载体添加到受弓形虫感染的人类巨噬细胞的细胞培养物中。以前已经通过猪肝酯酶的酶促降解研究了从载体中释放药物。通过透射测量，在UV / VIS光谱仪中研究了由于降解而导致的粒度减小。通过HPLC研究获得药物释放曲线。通过FACS（荧光激活细胞分选仪）记录了感染的人类巨噬细胞，弓形虫的种群动态以及模型药物载体的数量。作为主要结果，在2个系列的核壳模型药物载体中，无毒参考药物达到弓形虫病治疗效率观察值的约85％。这些数据与人巨噬细胞一侧的免疫刺激作用有关，这是由于人体摄取胶体底物对人体而言是细胞外源的。

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