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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Serine protease (TPS): A diagnostic and prognostic marker in pediatric patients with acute non-lymphoblastic leukemia
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Serine protease (TPS): A diagnostic and prognostic marker in pediatric patients with acute non-lymphoblastic leukemia

机译：丝氨酸蛋白酶（TPS）：小儿急性非淋巴细胞白血病的诊断和预后标志物

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The Serine protease, TPS (tryptase), is a specific marker for mast cells and mast cell-associated disorders. However, substantial amounts of TPS are also expressed in neoplastic myeloid, non-mast cell lineage. The aim of this study is determination and quantitation of TPS expression in patients with acute non-lymphoblastic leukemia (ANLL); to evaluate its prognostic value and its relevance as a genetic marker for detection of minimal residual blast cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect levels of TPS from 30 newly diagnosed ANLL children and 10 normal children served as controls. TPS levels for positive cases were reevaluated after induction chemotherapy; after onset of relapse or by the end of the study. Our results showed that the gene transcripts were detected in 56.7% of patients but were not expressed by normal controls. The highest frequency of TPS was recorded in patients with M4 showing significantly higher levels compared to other FAB (French–American–British Classification) subtypes. TPS levels were directly correlated to TLC, absolute blast counts in peripheral blood, levels of CD34 and CD117. After induction chemotherapy, levels of TPS decreased significantly in those who achieved complete remission while it increased significantly in relapsed patients, with a risk estimate of relapse six times higher in positive cases than TPS negative patients. 84.6% of the patients negative for TPS achieved complete remission with better disease free survival. In conclusion, TPS is expressed in a group of patients with ANLL thus serves as a disease related marker; it could be used as a prognostic indicator in evaluating remission status and early relapse as its elevated levels are associated with high risk of relapse; again, it is useful in predicting disease outcome.
机译：丝氨酸蛋白酶TPS（胰蛋白酶）是肥大细胞和肥大细胞相关疾病的特异性标记。但是，在肿瘤性髓样非肥大细胞系中也表达了大量的TPS。这项研究的目的是测定和定量急性非淋巴细胞白血病（ANLL）患者中TPS的表达;评估其预后价值及其作为检测最小残留母细胞的遗传标志物的相关性。逆转录聚合酶链反应（RT-PCR）用于检测30例新诊断的ANLL儿童和10例正常儿童的TPS水平。诱导化疗后重新评估阳性病例的TPS水平;复发后或研究结束时。我们的结果表明，在56.7％的患者中检测到了基因转录本，但正常对照组未表达。与其他FAB（法国-美国-英国-英国分类）亚型相比，M4患者的TPS最高记录。 TPS水平与TLC，外周血中绝对blast计数，CD34和CD117水平直接相关。诱导化疗后，完全缓解的患者中TPS的水平显着下降，而复发患者中的TPS水平则显着增加，阳性患者的复发风险估计是TPS阴性患者的6倍。 TPS阴性的患者中有84.6％实现了完全缓解，无病生存率更高。总之，TPS在一组ANLL患者中表达，因此可作为疾病相关的标志物。由于其升高的水平与复发的高风险相关，因此可以用作评估缓解状态和早期复发的预后指标;同样，它在预测疾病结局方面很有用。

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