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首页> 外文期刊>Iranian Journal of Reproductive Medicine >THE EFFECT OF ACTIVE IMMUNIZATION WITH GONADOTROPIN RELEASING HORMONE CONJUGATE (GNRH-BSA) ON GONADOSOMATIC INDICES (GSI) AND SPERM PARAMETERS IN MICE
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THE EFFECT OF ACTIVE IMMUNIZATION WITH GONADOTROPIN RELEASING HORMONE CONJUGATE (GNRH-BSA) ON GONADOSOMATIC INDICES (GSI) AND SPERM PARAMETERS IN MICE

机译：促性腺激素释放荷尔蒙共轭物（GNRH-BSA）的主动免疫对小鼠性激素指数（GSI）和精子参数的影响

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Background: Gonadotropin releasing hormone conjugate (GnRH-BSA) raises antibodies against biologically active gonadotropin releasing hormone (GnRH) which affects body weight, gonadosomatic indices (GSI) and sperm quality in male mice. Objective: The objective of this experimental investigation is to develop an effective and reliable hormonal immunocontraceptive vaccine to suppress spermatogenesis by using GnRH-BSA conjugate. Materials and Methods: Forty sexually mature mice, Mus musculus were divided into two groups of twenty each. Group 1, served as control, while group 2 were immunized at monthly intervals for four times against GnRH with a GnRH-BSA conjugate (50 mg) with aqueous adjuvant (Freund's adjuvant). After 30 days of each immunization, body weight, GSI and sperm quality were observed in the immunized groups and compared with the control group.Results: Body weight showed alterations in immunized animals as compared to control. However, GSI, sperm motility, sperm count and sperm morphology were significantly decreased in immunized animals throughout the experimental investigation and these effects were more prominent and significant in the later part of the experiment.Conclusion: These results suggested that the active immunization against GnRH produced bioeffective antibodies as indicated by significant reduction in GSI level and sperm quality and induced infertility in male mice.
机译：背景：促性腺激素释放激素偶联物（GnRH-BSA）产生抗生物活性促性腺激素释放激素（GnRH）的抗体，该激素会影响雄性小鼠的体重，促性腺激素指数（GSI）和精子质量。目的：本实验研究的目的是通过使用GnRH-BSA偶联物开发一种有效而可靠的激素免疫避孕疫苗来抑制精子发生。材料与方法：40只性成熟小鼠小家鼠分为两组，每组20只。第1组用作对照，而第2组用含水佐剂（弗氏佐剂）的GnRH-BSA缀合物（50mg）以每月间隔针对GnRH免疫四次。每次免疫30天后，观察免疫组的体重，GSI和精子质量，并与对照组进行比较。结果：免疫组动物的体重与对照组相比有所变化。然而，在整个实验研究中，免疫动物的GSI，精子活力，精子数量和精子形态显着下降，这些影响在实验后期更加突出和显着。结论：这些结果表明，针对GnRH的主动免疫产生了生物有效抗体，如雄性小鼠GSI水平和精子质量显着降低以及诱导的不育所表明。

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