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首页> 外文期刊>Iranian Journal of Reproductive Medicine >ASSESSMENT OF TESTIS HISTOLOGICAL CHANGES AND SPERM PARAMETERS IN EXPERIMENTALLYa€“INDUCED UNILATERAL AND BILATERAL CRYPTORCHID MOUSE MODEL
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ASSESSMENT OF TESTIS HISTOLOGICAL CHANGES AND SPERM PARAMETERS IN EXPERIMENTALLYa€“INDUCED UNILATERAL AND BILATERAL CRYPTORCHID MOUSE MODEL

机译：诱发单侧和双侧隐睾模型的实验中睾丸组织学变化和精子参数的评估

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Background: In most mammals, the testis is always maintained at a lower temperature than that in the abdomen, and exposure of the testis to body temperature causes degeneration of germ cells.Objective: In this research, the long effect of heat exposure on sperm parameters and microstructure of mouse testis were investigated. Cryptorchid mouse were induced by exposure to abdominal heat.Materials and Methods: Immature mice were anesthetized and a small incision was made in the abdominal skin, then fat pad at the upper end of testis was sutured to peritoneum. Weight of testis, spermatogenic cell numbers, tubular ectasis (rate of tubular lumen comparing to the thickness of germinal epithelium) as well as epididymal sperm parameters were measured.Results: The results showed that spermatogenesis was arrested and testicular weights, seminiferouse tubular diameters and epididymal sperm parameters were significantly reduced in the bilateral undescended testis compared with unilateral undescended testis and the control mice. However, complete depletion of seminiferous tubules and absence of germ cells was not found in the animals.Conclusion: In general, high temperature caused a decreased in all analyzed parameters except spermatogonial cell number probably due to the apoptosis and these changes significantly increase in bilateral groups compared with unilateral groups. We believe that the present model is a suitable tool for enrichment of spermatogonial stem cells, also it is useful for treatment of cryptorchidism and further biological research on spermatogenesis.
机译：背景：在大多数哺乳动物中，睾丸始终保持在低于腹部的温度下，睾丸暴露于体温会导致生殖细胞变性。目的：在这项研究中，长期暴露于热对精子参数的影响研究了小鼠睾丸的显微结构。材料和方法：麻醉未成熟的小鼠，在腹部皮肤上切一个小切口，然后将睾丸上端的脂肪垫缝合到腹膜上。测量睾丸重量，生精细胞数，肾小管扩张（肾小管腔腔率与生发上皮厚度的比率）以及附睾的精子参数。与单侧睾丸和对照组相比，双侧睾丸的精子参数显着降低。结论：一般情况下，高温导致除精原细胞数量外所有分析参数均下降，这可能是由于细胞凋亡引起的，而这些变化在两侧各组均明显增加。与单边团体相比。我们认为，本模型是富集精原干细胞的合适工具，也可用于治疗隐睾症和精子发生的进一步生物学研究。

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