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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Detection of occult HBV infection by nested PCR assay among chronic hepatitis C patients with and without hepatocellular carcinoma
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Detection of occult HBV infection by nested PCR assay among chronic hepatitis C patients with and without hepatocellular carcinoma

机译：巢式PCR法检测慢性丙型肝炎合并或非合并肝癌的隐匿性HBV感染

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Occult hepatitis B virus infection (OBI) has been reported among patients with chronic hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC). This study aimed to evaluate the prevalence of OBI in chronic hepatitis C patients with and without hepatocellular carcinoma. A total of 40 chronic hepatitis C patients who were negative for HBsAg, were enrolled into the study. They were classified into two groups: Group I which included 20 patients with chronic hepatitis C only whereas Group II included 20 patients with chronic hepatitis C and HCC. Detection of HBV DNA was done by nested-PCR using two sets of primers specific for surface and X viral genomes in serum and liver tissue of patients. Genotyping system based on PCR using type-specific primers was done for HBV-DNA positive intrahepatic samples. OBI was detected in the liver tissue in 23/40 (57.5%) of chronic HCV infected patients, 18 (78.3%) of whom belonged to group II, conferring a 90% prevalence of this group. In serum, HBV-DNA was detected in 9/40 patients (22.5%), 7 (77.8%) of whom belonged to group II. Among the 23 positive intrahepatic HBV-DNA samples studied, HBV-genotype D (34.8%) and B (26.1%) were more predominant, whereas HBV-genotype C (13.1%) and A (8.7%) infections were the least observed, respectively; mixed genotypes were detected in 17.4% ( n =4), 3 of them were with HBV-genotype D and B, and 1 was with HBV-genotype C and A. In conclusion OBI is a fact in our community; it was detected in liver tissue of chronic HCV-infected patients, especially in cases of chronic HCC. In addition, OBI might be related to severity of necroinflammatory activity and fibrosis. Large studies are needed to confirm that co-infection could determine a worse progress of chronic liver disease in this population. Also, detection of intrahepatic HBV-DNA is more useful in diagnosis of OBI.
机译：慢性丙型肝炎病毒（HCV）感染和肝细胞癌（HCC）的患者中已报告隐匿性乙型肝炎病毒感染（OBI）。这项研究旨在评估OBI在患有和不患有肝细胞癌的慢性丙型肝炎患者中的患病率。该研究共纳入40例HBsAg阴性的慢性丙型肝炎患者。他们分为两组：第一组仅包括20例慢性丙型肝炎患者，第二组包括20例慢性丙型肝炎和HCC患者。 HBV DNA的检测是通过嵌套PCR使用两组特异性针对患者血清和肝组织中表面和X病毒基因组的引物进行的。对HBV-DNA阳性肝内样品进行了基于PCR的基因分型系统，该PCR使用类型特异性引物。在23/40（57.5％）慢性HCV感染的患者的肝组织中检测到OBI，其中18（78.3％）属于第二组，占这一组的90％患病率。在血清中，在9/40例患者（22.5％）中检测到HBV-DNA，其中7例（77.8％）属于II组。在研究的23例阳性肝内HBV-DNA样本中，HBV基因型D（34.8％）和B（26.1％）更为主要，而HBV基因型C（13.1％）和A（8.7％）感染最少，分别;混合基因型的检出率为17.4％（n = 4），其中3例是HBV基因型D和B，1例是HBV基因型C和A。在慢性HCV感染患者的肝脏组织中检测到了它，尤其是在慢性HCC病例中。此外，OBI可能与坏死性炎症活动和纤维化的严重程度有关。需要进行大量研究以确认共同感染可以确定该人群中慢性肝病的恶化情况。同样，肝内HBV-DNA的检测对OBI的诊断更为有用。

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