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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >The antiproliferative effect of mulberry ( Morus alba L.) plant on hepatocarcinoma cell line HepG2
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The antiproliferative effect of mulberry ( Morus alba L.) plant on hepatocarcinoma cell line HepG2

机译：桑树（ Morus alba L.）植物对肝癌细胞HepG2的抗增殖作用

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This study aimed to investigate the antiproliferative effect of aqueous and organic extracts of mulberry leaves ( Morus Alba L.) on human hepatocellular carcinoma HepG2 cell line. Mulberry leaf extracts were prepared using the solvents: water, 50% aqueous MeOH, and 100% MeOH for different time intervals, while the cells treated with dimethyl sulfoxide (DMSO) served as control. The effects of aqueous and organic extracts of M. alba L. leaves on HepG2 cell viability, nuclear factor kappa B (NF-κB) gene expression, alfa-fetoprotein (AFP), albumin (ALB), gamma-glutamyl transpeptidase (γ-GT) and alkaline phosphatase (ALP) were measured. The results of the cell viability assays showed that water, 50% aqueous MeOH, and 100% MeOH extracts exhibited a highly significant inhibitory effect on HepG2 cell proliferation which was evidenced by a reduction in viable cell count. The results were confirmed by microscopical examination of cell morphology. Furthermore, the mulberry leaf extracts suppressed the activity of NF-κB gene expression of HepG2 cells compared to the control. Also a highly significant depression occurred at the levels of AFP, γ-GT and ALP in HepG2 cells compared with that of controls in a time dependent manner. By contrast, the mulberry leaf extracts increased the secretion of ALB. Therefore, the conclusion was that the organic and aqueous extracts of mulberry leaves inhibit the growth of HepG2 cells through suppressing the activity of NF-κB gene expression and modulate the biochemical markers.
机译：这项研究旨在研究桑叶的水提取物和有机提取物（桑属（Morus Alba L.））对人肝癌HepG2细胞系的抗增殖作用。使用不同时间间隔的溶剂：水，50％MeOH水溶液和100％MeOH制备桑叶提取物，而用二甲基亚砜（DMSO）处理的细胞作为对照。白花苜蓿叶水提取物和有机提取物对HepG2细胞活力，核因子κB（NF-κB）基因表达，甲胎蛋白（AFP），白蛋白（ALB），γ-谷氨酰转肽酶（γ- GT）和碱性磷酸酶（ALP）进行了测量。细胞活力测定的结果表明，水，50％的MeOH水溶液和100％的MeOH提取物对HepG2细胞的增殖表现出高度显着的抑制作用，这通过减少活细胞计数来证明。通过显微镜检查细胞形态证实了该结果。而且，与对照相比，桑叶提取物抑制了HepG2细胞的NF-κB基因表达活性。与对照组相比，HepG2细胞中AFP，γ-GT和ALP的水平也发生了高度显着的抑制，并呈时间依赖性。相反，桑叶提取物增加了ALB的分泌。因此，得出的结论是，桑叶的有机提取物和水提取物通过抑制NF-κB基因表达的活性并调节生化标志物来抑制HepG2细胞的生长。

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