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首页> 外文期刊>Electronic Journal of Biotechnology >Betaine supplementation improved l-threonine fermentation of Escherichia coli THRD by upregulating zwf (glucose-6-phosphate dehydrogenase) expression
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Betaine supplementation improved l-threonine fermentation of Escherichia coli THRD by upregulating zwf (glucose-6-phosphate dehydrogenase) expression

机译：添加甜菜碱可通过上调 zwf 大肠杆菌 THRD的 l -苏氨酸发酵>（6-磷酸葡萄糖脱氢酶）表达

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BackgroundThe supplementation of betaine, an osmoprotective compatible solute, in the cultivation media has been widely used to protect bacterial cells. To explore the effects of betaine addition on industrial fermentation,Escherichia coliTHRD, anl-threonine producer, was used to examine the production ofl-threonine with betaine supplementation and the underlying mechanism through which betaine functions was investigated.ResultsBetaine supplementation in the medium ofE.coliTHRD significantly improvedl-threonine fermentation parameters. The transcription ofzwfand corresponding enzyme activity of glucose-6-phosphate dehydrogenase were significantly promoted by betaine addition, which contributed to an enhanced expression ofzwfthat provided more nicotinamide adenine dinucleotide phosphate (NADPH) forl-threonine synthesis. In addition, as a result of the betaine addition, the betaine-stimulated expression of enhanced green fluorescent protein (eGFP) under thezwfpromoter within a plasmid-based cassette proved to be a transcription-level response ofzwf. Finally, the promoter of the phosphoenolpyruvate carboxylase geneppcin THRD was replaced with that ofzwf, whilel-threonine fermentation of the new strain was promoted by betaine addition.ConclusionsWe reveal a novel mode of betaine that facilitates the microbial production of useful compounds. Betaine supplementation upregulates the expression ofzwfand increases the NADPH synthesis, which may be beneficial for the cell growth and thereby promote the production ofl-threonine. This finding might be useful for the production of NADPH-dependent amino acids and derivatives inE.coliTHRD or otherE.colistrains.How to cite:Li Y, Zhang D, Cai N, et al. Betaine supplementation improvedl-threonine fermentation ofEscherichia coliTHRD by upregulatingzwf(glucose-6-phosphate dehydrogenase) expression. Electron J Biotechnol 2019;39.https://doi.org/10.1016/j.ejbt.2019.03.004.
机译：背景技术在培养基中补充甜菜碱（一种具有渗透保护作用的溶质）已被广泛用于保护细菌细胞。为了研究甜菜碱添加对工业发酵的影响，使用了α-苏氨酸生产者大肠杆菌THRD来检查补充甜菜碱的1-苏氨酸的生产以及研究甜菜碱功能的基本机理。结果在大肠杆菌THRD培养基中补充甜菜碱苏氨酸的发酵参数明显改善。甜菜碱的加入显着促进了6-葡萄糖磷酸脱氢酶的zwf转录和相应的酶活性，这有助于zwf的表达增强，从而提供了更多的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）甲酸-苏氨酸合成。另外，由于甜菜碱的添加，在基于质粒的盒中的zwf启动子下，甜菜碱刺激的增强型绿色荧光蛋白（eGFP）的表达被证明是zwf的转录水平应答。最后，磷酸烯醇丙酮酸羧化酶基因启动子THRD的启动子被zwf取代，同时通过添加甜菜碱促进了新菌株的1-苏氨酸发酵。结论我们揭示了一种新型的甜菜碱模式，该模式促进了有用化合物的微生物生产。甜菜碱补充上调zwfand的表达，增加NADPH的合成，这可能对细胞生长有利，从而促进1-苏氨酸的产生。这一发现可能对在大肠杆菌THRD或其他大肠杆菌中产生NADPH依赖性氨基酸及其衍生物有用。甜菜碱的补充通过上调zwf（6-磷酸葡萄糖脱氢酶）的表达来改善大肠杆菌THRD的1-苏氨酸发酵。 Electron J生物技术杂志2019; 39.https：//doi.org/10.1016/j.ejbt.2019.03.004。

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