Ellagic acid and ellagitannins are defense phytochemicals and dietary polyphenols found in fruits and nuts and implicated with strong antioxidant, anticancer and antiatherosclerotic biological properties. Unfortunately, there are few reports on the bioconversion studies from ellagitannins to ellagic acid. The objective of the present work was to use of partially purified ellagitannins from pomegranate peels as inducers for production of ellagic acid by Aspergillus niger GH1 in solid-state fermentation on inert supports (perlite, polyurethane foam and nylon fiber). For total pomegranate polyphenols (TPP), two methodologies were compared. Higher recovery of TTP (70 g kg-1 peel) in a short time (2 h) were obtained by filtering through a muslin cloth followed by centrifugation at 3000 rpm for 15 min. Production of ellagic acid and ellagitannase enzyme were evaluated. Maximum ellagic acid production (231.22 mg g-1 TPP) at 24 h cultivation with an ellagitannase activity of 3213 U L-1 was obtained when using polyurethane foam as inert support. Polyurethane foam was the best solid support to produce high yields of ellagic acid production.
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机译：鞣花酸和鞣花单宁是水果和坚果中的防御性植物化学物质和膳食多酚，具有强大的抗氧化剂，抗癌和抗动脉粥样硬化生物学特性。不幸的是，关于从鞣花单宁到鞣花酸的生物转化研究的报道很少。本工作的目的是使用从石榴皮中提纯的部分鞣花单宁作为诱导剂，以黑曲霉GH1在惰性载体（珍珠岩，聚氨酯泡沫和尼龙纤维）上进行固态发酵生产鞣花酸。对于总石榴多酚（TPP），比较了两种方法。通过用细棉布过滤，然后在3000 rpm下离心15分钟，可以在短时间内（2小时）获得更高的TTP回收率（70 g kg-1果皮）。评价鞣花酸和鞣花酶的产生。当使用聚氨酯泡沫作为惰性载体时，在24小时培养时具有最大的鞣花酸产量（231.22 mg g-1 TPP），其鞣花聚糖酶活性为3213 U L-1。聚氨酯泡沫是产生高产鞣花酸的最佳固体载体。
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