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首页> 外文期刊>Endoscopy International Open >Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP)
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Residual bioburden in reprocessed side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP)

机译：内窥镜逆行胰胆管造影（ERCP）的后视镜内窥镜中残留的生物负荷

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Background and study aim: Worldwide some endoscopy units routinely continue to use manual reprocessing techniques for disinfection of side-view endoscopes. The aim of this study was to evaluate the outcome quality of manual reprocessing techniques for removal and inactivation of the bioburden from side-view endoscopes used for endoscopic retrograde cholangiopancreatography (ERCP) in a tertiary referral endotherapy unit in Sri Lanka. Methods: 102 samples obtained from two different flexible side-view endoscopes (Olympus TJF Q 180V and Olympus TJF 160?R) were tested for microbial growth. Three samples were collected each time; one swab from the tip before and another after manual reprocessing. The third sample was collected by flushing the working channel with sterile normal saline after manual reprocessing. Microorganisms were identified by culturing the samples. Result:: After reprocessing, culture-positive rates were 20?% and 9?% for the samples obtained from the tip and the working channel of the side-view endoscopes, respectively. Klebsiella spp.?and Candida spp.?were found to be the commonest microorganisms in the samples from the tips and from the working channels, respectively, of the reprocessed side-view endoscopes. Conclusion: There is a high culture-positive rate after reprocessing of the side-view endoscopes using the manual reprocessing procedure, despite strict adherence to the protocol for reprocessing.
机译：背景和研究目标：在世界范围内，某些内窥镜检查单位通常继续使用手动后处理技术对侧视内窥镜进行消毒。这项研究的目的是评估在斯里兰卡三级转诊内窥镜治疗单元中用于内窥镜逆行胰胆管造影术（ERCP）的侧视内窥镜去除和灭活生物负荷的人工后处理技术的结果质量。方法：测试了从两个不同的柔性侧视内窥镜（Olympus TJF Q 180V和Olympus TJF 160？R）获得的102个样品的微生物生长情况。每次收集三个样品。手动处理之前，先从尖端擦拭一次，然后再进行手工处理。手动处理后，通过用无菌生理盐水冲洗工作通道来收集第三份样品。通过培养样品鉴定微生物。结果：：后处理后，从侧视图和内窥镜工作通道获得的样品的培养阳性率分别为20％和9％。分别从后处理的内窥镜的尖端和工作通道中发现，克雷伯菌属和念珠菌属是样品中最常见的微生物。结论：尽管严格遵守后处理协议，但使用手动后处理程序对侧视内窥镜进行后处理后，培养阳性率较高。

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