• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Epigenetics & Chromatin >HP1γ function is required for male germ cell survival and spermatogenesis
【24h】

HP1γ function is required for male germ cell survival and spermatogenesis

机译：HP1γ功能是雄性生殖细胞存活和生精所必需的

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background HP1 proteins are conserved components of eukaryotic constitutive heterochromatin. In mammals, there are three genes that encode HP1-like proteins, termed HP1α, HP1β and HP1γ, which have a high degree of homology This paper describes for the first time, to our knowledge, the physiological function of HP1γ using a gene-targeted mouse. Results While targeting the Cbx3 gene (encoding the HP1γ protein) with a conditional targeting vector, we generated a hypomorphic allele (Cbx3hypo), which resulted in much reduced (barely detectable) levels of HP1γ protein. Homozygotes for the hypomorphic allele (Cbx3hypo/hypo) are rare, with only 1% of Cbx3hypo/hypo animals reaching adulthood. Adult males exhibit a severe hypogonadism that is associated with a loss of germ cells, with some seminiferous tubules retaining only the supporting Sertoli cells (Sertoli cell-only phenotype). The percentage of seminiferous tubules that are positive for L1 ORF1 protein (ORF1p) in Cbx3hypo/hypo testes is greater than that for wild-type testes, indicating that L1 retrotransposon silencing is reversed, leading to ectopic expression of ORF1p in Cbx3hypo/hypo germ cells. Conclusions The Cbx3 gene product (the HP1γ protein) has a non-redundant function during spermatogenesis that cannot be compensated for by the other two HP1 isotypes. The Cbx3hypo/hypo spermatogenesis defect is similar to that found in Miwi2 and Dnmt3L mutants. The Cbx3 gene-targeted mice generated in this study provide an appropriate model for the study of HP1γ in transposon silencing and parental imprinting.
机译：背景HP1蛋白是真核组成型异染色质的保守成分。在哺乳动物中，有三种编码HP1样蛋白的基因，分别称为HP1α，HP1β和HP1γ，它们具有高度的同源性。本文据我们所知首次描述了以基因为靶点的HP1γ的生理功能。老鼠。结果用条件靶向载体靶向Cbx3基因（编码HP1γ蛋白）时，我们产生了一个亚型等位基因（Cbx3 hypo ），导致HP1γ蛋白水平大大降低（几乎检测不到）。亚型等位基因（Cbx3 hypo / hypo ）的纯合子很少见，只有1％的Cbx3 hypo / hypo 动物成年。成年男性表现出严重的性腺机能减退，这与生殖细胞的丧失有关，一些生精小管仅保留支持性支持细胞（仅支持Sertoli的表型）。 Cbx3 hypo / hypo 睾丸中L1 ORF1蛋白（ORF1p）阳性的曲细精管百分比高于野生型睾丸，表明L1逆转座子沉默被逆转，导致异位表达Cbx3 hypo / hypo 生殖细胞中ORF1p的表达。结论Cbx3基因产物（HP1γ蛋白）在精子发生过程中具有非冗余功能，其他两种HP1亚型无法弥补这一功能。 Cbx3 hypo / hypo 精子发生缺陷类似于在Miwi2和Dnmt3L突变体中发现的缺陷。在这项研究中生成的针对Cbx3基因的小鼠为转座子沉默和亲子印迹研究HP1γ提供了合适的模型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号