• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Epigenetics & Chromatin >An in vitro-identified high-affinity nucleosome-positioning signal is capable of transiently positioning a nucleosome in vivo
【24h】

An in vitro-identified high-affinity nucleosome-positioning signal is capable of transiently positioning a nucleosome in vivo

机译：体外鉴定的高亲和力核小体定位信号能够在体内瞬时定位核小体

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The physiological function of eukaryotic DNA occurs in the context of nucleosomal arrays that can expose or obscure defined segments of the genome. Certain DNA sequences are capable of strongly positioning a nucleosome in vitro, suggesting the possibility that favorable intrinsic signals might reproducibly structure chromatin segments. As high-throughput sequencing analyses of nucleosome coverage in vitro and in vivo have become possible, a vigorous debate has arisen over the degree to which intrinsic DNA:nucleosome affinities orchestrate the in vivo positions of nucleosomes, thereby controlling physical accessibility of specific sequences in DNA. Results We describe here the in vivo consequences of placing a synthetic high-affinity nucleosome-positioning signal, the 601 sequence, into a DNA plasmid vector in mice. Strikingly, the 601 sequence was sufficient to position nucleosomes during an early phase after introduction of the DNA into the mice (when the plasmid vector transgene was active). This positioning capability was transient, with a loss of strong positioning at a later time point when the transgenes had become silent. Conclusions These results demonstrate an ability of DNA sequences selected solely for nucleosome affinity to organize chromatin in vivo, and the ability of other mechanisms to overcome these interactions in a dynamic nuclear environment.
机译：背景真核DNA的生理功能发生在核小体阵列的背景下，核小体阵列可暴露或模糊基因组的定义片段。某些DNA序列能够在体外强烈定位核小体，这表明有利的内在信号可能可再现地构造染色质片段的可能性。随着对体外和体内核小体覆盖率的高通量测序分析成为可能，关于固有DNA：核小体亲和力协调核小体在体内位置从而控制DNA中特定序列的物理可及性的程度引起了激烈的争论。结果我们在此处描述了将合成的高亲和力核小体定位信号601序列置于小鼠的DNA质粒载体中的体内后果。令人惊讶的是，在将DNA导入小鼠体内后（质粒载体转基因有活性时），601序列足以在早期定位核小体。这种定位能力是暂时的，在转基因变得沉默后的稍后时间点失去了强大的定位。结论这些结果证明了仅针对核小体亲和力选择的DNA序列在体内组织染色质的能力，以及在动态核环境中克服这些相互作用的其他机制的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号