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首页> 外文期刊>African Journal of Microbiology Research >Evaluation of culture media for selective enumeration of Bifidobacterium spp. in combination with different strains of Streptococcus thermophilus isolated from commercial yogurt starter cultures
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Evaluation of culture media for selective enumeration of Bifidobacterium spp. in combination with different strains of Streptococcus thermophilus isolated from commercial yogurt starter cultures

机译：评估培养基对双歧杆菌属物种的选择性计数。与从商业酸奶发酵剂培养物中分离得到的不同菌株嗜热链球菌联合使用

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摘要

Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus are involved in the manufacture of yogurt, and Bifidobacterium spp. can be supplemented if probiotic properties are expected. In such products, enumeration of each bacterial component is a hard task since they have similar nutritional requirements. For this reason, there is still no official and reliable methodology for this purpose. The objectives of the present study were to evaluate the most appropriate culture medium for the enumeration of four different Bifidobacterium species in the presence of S. thermophilus and L. delbrueckii subsp. bulgaricus isolated from commercial cultures and investigate the influence of different strains of S. thermophilus on the choice of medium. Among 11 isolates of S. thermophilus obtained from these cultures, and which were submitted to repetitive element sequence-based PCR (rep-PCR), nine were found to be distinct strains. For selective enumeration of the Bifidobacterium species, results were influenced by the S. thermophilus strain used, but De Man, Rogosa and Sharpe (MRS) agar containing bile salts showed the best results for all of the Bifidobacterium species.
机译：嗜热链球菌和德氏乳杆菌亚种。保加利亚人参与制造酸奶和双歧杆菌。如果期望益生菌特性，可以补充。在此类产品中，由于细菌具有相似的营养要求，因此每个细菌成分的计数都是一项艰巨的任务。因此，目前尚无官方和可靠的方法用于此目的。本研究的目的是评估在嗜热链球菌和德氏乳杆菌亚种存在的情况下，对四种不同的双歧杆菌物种进行计数的最合适培养基。保加利亚保加利亚从商业文化中分离出来，研究嗜热链球菌不同菌株对培养基选择的影响。在从这些培养物中获得的11株嗜热链球菌分离株中，并进行了基于重复元件序列的PCR（rep-PCR），发现其中9株是不同的菌株。对于双歧杆菌物种的选择性枚举，结果受所用嗜热链球菌菌株的影响，但是含有胆汁盐的De Man，Rogosa和Sharpe（MRS）琼脂显示了所有双歧杆菌物种的最佳结果。

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