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首页> 外文期刊>African Journal of Microbiology Research >Effects of different levels of phosphate in comparison with citrate buffer in culture media on in vitro ruminal fermentation and methanogenesis of a starch-rich feed mixture
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Effects of different levels of phosphate in comparison with citrate buffer in culture media on in vitro ruminal fermentation and methanogenesis of a starch-rich feed mixture

机译：与培养基中柠檬酸盐缓冲液相比，不同水平的磷酸盐对瘤胃发酵和富含淀粉的饲料混合物甲烷生成的影响

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Compared to a phosphate free medium containing citrate buffer, effects of phosphate concentrations (10, 20, 50 ,and 100 mmol/L) in the modified Menke and Steingass’s medium on methanogenesis of a maize-rich substrate (Leymus chinensis?hay:maize meal = 1:4) were determined in comparison with using an?in vitro?cumulative gas production technique. After the 48 h batch cultures, phosphate additions decreased total gas production (GP48, ml/g dry matter (DM)) (P < 0.05) as well as the gas production rate to reach maximum digestion (RmaxG, mL/h) (P < 0.01). Total volatile fatty acids (VFA) concentrations (mmol/L) were decreased by 28%, while the phosphate concentration increased up to 50 to 100 mmol/L (P < 0.05). Volatile fatty acid production shifted from an initially acetate dominated production towards a propionate production as demonstrated by the ratio of non-glucogenic to glucogenic acids (NGR) (P < 0.01). Although, the molar proportions of CO2, CH4, and H2?in the fermentation gases were not altered, the net fractional CH4?production (ml/g DM) in the phosphate levels of 10, 20, 50, and 100 mmol/L were reduced by 6.2, 11.8, 18.57, and 26.2%. The results obtained in the present study showed that a phosphate-mediated reduction in methane emissions occurred without the expense of a reduction in the digestion of the substrate, and the mechanism that phosphate inhibited acetoclastic methanogenesis on rice roots may exist in the rumen.
机译：与含柠檬酸盐缓冲液的无磷酸盐培养基相比，改良的Menke和Steingass培养基中的磷酸盐浓度（10、20、50和100 mmol / L）对富含玉米的底物（羊草（Leymus chinensis？hay：hay粕））产甲烷的影响= 1：4）与使用体外累积气体生产技术进行比较。分批培养48小时后，添加磷酸盐会降低总产气量（GP48，ml / g干物质（DM））（P <0.05），以及产气速率达到最大消化率（RmaxG，mL / h）（P <0.01）。总挥发性脂肪酸（VFA）浓度（mmol / L）降低了28％，而磷酸盐浓度升高到50至100 mmol / L（P <0.05）。非糖原酸与糖原酸（NGR）之比表明，挥发性脂肪酸的生产从最初的乙酸盐为主的生产转向丙酸的生产（P <0.01）。尽管发酵气体中的CO2，CH4和H2α的摩尔比例没有改变，但是磷酸盐水平为10、20、50和100 mmol / L时，CH4α的净生产分数（ml / g DM）却有所变化。减少了6.2、11.8、18.57和26.2％。在本研究中获得的结果表明，在不降低底物消化的代价的情况下，发生了磷酸盐介导的甲烷排放量的降低，而且瘤胃中可能存在磷酸盐抑制稻根上破土作用的机制。

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