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首页> 外文期刊>European Cells & Materials >In situ vascular regeneration using substance P-immobilised poly(L-lactide-co-ε-caprolactone) scaffolds: stem cell recruitment, angiogenesis, and tissue regeneration
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In situ vascular regeneration using substance P-immobilised poly(L-lactide-co-ε-caprolactone) scaffolds: stem cell recruitment, angiogenesis, and tissue regeneration

机译：使用固定有P物质的聚（L-丙交酯-co-ε-己内酯）支架进行原位血管再生：干细胞募集，血管生成和组织再生

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In situ tissue regeneration holds great promise for regenerative medicine and tissue engineering applications. However, to achieve control over long-term and localised presence of biomolecules, certain barriers must be overcome. The aim of this study was to develop electrospun scaffolds for the fabrication of artificial vascular grafts that can be remodelled within a host by endogenous cell recruitment. We fabricated scaffolds by mixing appropriate proportions of linear poly (l-lactide-co-ε-caprolactone) (PLCL) and substance P (SP)-immobilised PLCL, using electrospinning to develop vascular grafts. Substance P was released in a sustained fashion from electrospun membranes for up to 30 d, as revealed by enzyme-linked immunosorbent assay. Immobilised SP remained bioactive and recruited human bone marrow-derived mesenchymal stem cells (hMSCs) in an in vitro Trans-well migration assay. The biocompatibility and biological performance of the scaffolds were evaluated by in vivo experiments involving subcutaneous scaffold implantations in Sprague-Dawley rats for up to 28 d followed by histological and immunohistochemical studies. Histological analysis revealed a greater extent of accumulative host cell infiltration and collagen deposition in scaffolds containing higher contents of SP than observed in the control group at both time points. We also observed the presence of a large number of laminin-positive blood vessels and Von Willebrand factor (vWF+) cells in the explants containing SP. Additionally, scaffolds containing SP showed the existence of CD90+ and CD105+ MSCs. Collectively, these findings suggest that the methodology presented here may have broad applications in regenerative medicine, and the novel scaffolding materials can be used for in situ tissue regeneration of soft tissues.
机译：原位组织再生在再生医学和组织工程应用中具有广阔的前景。但是，要实现对生物分子长期和局部存在的控制，必须克服某些障碍。这项研究的目的是开发用于制造人造血管移植物的电纺支架，该移植物可以在宿主内通过内源性细胞募集而重塑。我们通过混合适当比例的线性聚（l-丙交酯-co-ε-己内酯）（PLCL）和固定有P（SP）的PLCL制备支架，使用静电纺丝技术开发血管移植物。酶联免疫吸附试验表明，P物质从电纺膜中持续释放长达30 d。固定的SP仍具有生物活性，并在体外Trans-well迁移测定中募集了人类骨髓来源的间充质干细胞（hMSC）。支架的生物相容性和生物学性能通过体内实验评估，该实验涉及在Sprague-Dawley大鼠中皮下植入支架长达28天，然后进行组织学和免疫组织化学研究。组织学分析显示，在两个时间点上，与对照组相比，含SP含量更高的支架中的宿主细胞浸润和胶原蛋白沉积的程度更大。我们还观察到含有SP的外植体中存在大量层粘连蛋白阳性血管和Von Willebrand因子（vWF +）细胞。另外，含有SP的支架显示出CD90 +和CD105 + MSC的存在。总而言之，这些发现表明，本文介绍的方法在再生医学中可能具有广泛的应用，并且新型支架材料可用于软组织的原位组织再生。

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