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首页> 外文期刊>European Cells & Materials >Biological and bactericidal properties of Ag-doped bioactive glass in a natural extracellular matrix hydrogel with potential application in dentistry
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Biological and bactericidal properties of Ag-doped bioactive glass in a natural extracellular matrix hydrogel with potential application in dentistry

机译：天然细胞外基质水凝胶中掺银生物活性玻璃的生物和杀菌特性及其在牙科中的潜在应用

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The aim of this study was the fabrication and evaluation of a novel bioactive and bactericidal material, which could have applications in dentistry by supporting tissue regeneration and killing oral bacteria. Our hypothesis was that a new scaffold for pulp-dentin tissue engineering with enhanced antibacterial activity could be obtained by associating extracellular matrix derived from porcine bladder with an antibacterial bioactive glass. Our study combines in vitro approaches and ectopic implantation in scid mice. The novel material was fabricated by incorporating a sol-gel derived silver (Ag)-doped bioactive glass (BG) in a natural extracellular matrix (ECM) hydrogel in ratio 1:1 in weight % (Ag-BG/ECM). The biological properties of the Ag-BG/ECM were evaluated in culture with dental pulp stem cells (DPSCs). In particular, cell proliferation, cell apoptosis, stem cells markers profile, and cell differentiation potential were studied. Furthermore, the antibacterial activity against Streptococcus mutans and Lactobacillus casei was measured. Moreover, the capability of the material to enhance pulp/dentin regeneration in vivo was also evaluated. Our data show that Ag-BG/ECM significantly enhances DPSCs’ proliferation, it does not affect cell morphology and stem cells markers profile, protects cells from apoptosis, and enhances in vitro cell differentiation and mineralisation potential as well as in vivo dentin formation. Furthermore, Ag-BG/ECM strongly inhibits S. mutans and L. casei growth suggesting that the new material has also anti-bacterial properties. This study provides foundation for future clinical applications in dentistry. It could potentially advance the currently available options of dental regenerative materials.
机译：这项研究的目的是制造和评估一种新型的生物活性和杀菌材料，该材料可通过支持组织再生和杀死口腔细菌而应用于牙科领域。我们的假设是，通过将猪膀胱来源的细胞外基质与抗菌生物活性玻璃结合，可以得到一种具有增强抗菌活性的用于牙髓-牙本质组织工程的新型支架。我们的研究结合了scid小鼠的体外方法和异位植入。通过将溶胶-凝胶衍生的掺银（Ag）的生物活性玻璃（BG）掺入天然细胞外基质（ECM）水凝胶中，重量比为1：1（Ag-BG / ECM），从而制造出这种新型材料。在牙髓干细胞（DPSC）培养中评估了Ag-BG / ECM的生物学特性。特别地，研究了细胞增殖，细胞凋亡，干细胞标志物谱和细胞分化潜力。此外，测定了对变形链球菌和干酪乳杆菌的抗菌活性。此外，还评估了该材料增强体内牙髓/牙本质再生的能力。我们的数据表明，Ag-BG / ECM显着增强DPSC的增殖，不影响细胞形态和干细胞标志物分布，保护细胞免于凋亡，并增强体外细胞分化和矿化潜力以及体内牙本质的形成。此外，Ag-BG / ECM强烈抑制变形链球菌和干酪乳杆菌的生长，表明该新材料还具有抗菌性能。这项研究为牙科的未来临床应用提供了基础。它可能会推动牙科再生材料的当前可用选择。

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