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首页> 外文期刊>European Journal of Histochemistry >Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
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Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts

机译：分化破骨细胞膜结合RANKL的网格蛋白依赖性内吞作用。

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摘要

Bone is continuously repaired and remodelled through well-coordinated activity of osteoblasts that form new bone and osteoclasts, which resorb it. Osteoblasts synthesize and secrete two key molecules that are important for osteoclast differentiation, namely the ligand for the receptor of activator of nuclear factor κB (RANKL) and its decoy receptor osteoprotegerin (OPG). Active membrane transport is a typical feature of the resorbing osteoclast during bone resorption. Normally, one resorption cycle takes several hours as observed by monitoring actin ring formation and consequent disappearance in vitro. During these cyclic changes, the cytoskeleton undergoes remarkable dynamic rearrangement. Active cells show a continuous process of exocytosis that plays an essential role in transport of membrane components, soluble molecules and receptor-mediated ligands thus allowing them to communicate with the environment. The processes that govern intracellular transport and trafficking in mature osteoclasts are poorly known. The principal methodological problem that have made these studies difficult is a physiological culture of osteoclasts that permit observing the vesicle apparatus in conditions similar to the in vivo conditions. In the present study we have used a number of morphological approaches to characterize the composition, formation and the endocytic and biosynthetic pathways that play roles in dynamics of differentiation of mature bone resorbing cells using a tri-dimensional system of physiologic coculture.
机译：骨骼通过成骨细胞的良好协调活动不断修复和重塑，形成新的骨骼和破骨细胞，再吸收骨骼。成骨细胞合成并分泌对破骨细胞分化至关重要的两个关键分子，即核因子κB激活剂受体的配体（RANKL）和诱饵受体骨保护素（OPG）。主动的膜运输是骨吸收过程中吸收破骨细胞的典型特征。正常情况下，通过监测肌动蛋白环的形成及其在体外的消失，一个吸收周期需要几个小时。在这些循环变化过程中，细胞骨架会发生显着的动态重排。活性细胞显示出胞吐作用的连续过程，在膜成分，可溶性分子和受体介导的配体的运输中起着至关重要的作用，从而使它们与环境相通。控制成熟破骨细胞的细胞内运输和运输的过程知之甚少。使这些研究变得困难的主要方法学问题是破骨细胞的生理学培养，其允许在类似于体内条件的条件下观察囊泡装置。在本研究中，我们已使用多种形态学方法来表征组成，形成以及内吞和生物合成途径，这些途径在使用生理共培养的三维系统的成熟骨吸收细胞分化动力学中起作用。

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