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首页> 外文期刊>European Journal of Histochemistry >The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study
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The cytoskeleton of chondrocytes of Sepia officinalis (Mollusca, Cephalopoda): an immunocytochemical study

机译:乌贼墨软骨细胞的细胞骨架:一项免疫细胞化学研究

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摘要

Our previous electron microscope study showed that chondrocytes from cephalopod cartilage possess a highly developed cytoskeleton and numerous cytoplasmic processes that ramify extensively through the tissue. We have now carried out a light microscope immunocytochemical study of chondrocytes from the orbital cartilage of Sepia officinalis to obtain indications as to the nature of the cytoskeletal components. We found clear positivity to antibodies against mammalian tubulin, vimentin, GFAP, and actin, but not keratin. The simultaneous presence of several cytoskeletal components is consistent with the hypothesis that cephalopod chondrocytes have the characteristics of both chondrocytes and osteocytes of vertebrates, which endow the tissue as a whole with some of the properties of vertebrate bone. We confirm, therefore, the presence in molluscs of the ubiquitous cytoskeletal proteins of metazoan cells that have remained highly conserved throughout phylogenetic evolution.
机译:我们以前的电子显微镜研究表明,头足软骨的软骨细胞具有高度发达的细胞骨架和许多在整个组织中广泛分布的胞质过程。现在,我们已经对乌贼墨眼眶软骨中的软骨细胞进行了光学显微镜免疫细胞化学研究,以了解细胞骨架成分的性质。我们发现对哺乳动物微管蛋白,波形蛋白,GFAP和肌动蛋白的抗体有明显的阳性,但对角蛋白没有阳性。几种细胞骨架成分的同时存在与以下假设相符:头足类软骨细胞具有脊椎动物的软骨细胞和骨细胞的特征,从而使整个组织具有脊椎动物骨的某些特性。因此,我们确认,在软体动物中存在后生动物细胞的遍在细胞骨架蛋白,这些蛋白在整个系统发育进化中一直高度保守。

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