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首页> 外文期刊>European Journal of Histochemistry >Fate of (D-Ala2)-deltorphin-I-like immunoreactive neurons in 6-hydroxydopamine lesioned rat brain
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Fate of (D-Ala2)-deltorphin-I-like immunoreactive neurons in 6-hydroxydopamine lesioned rat brain

机译：（D-Ala2）-deltorphin-I样免疫反应神经元在6-羟基多巴胺损伤大鼠脑中的命运

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摘要

The use of a polyclonal antiserum specific to C-terminal tetrapeptide amide of (D-Ala2)deltorphin-I, a naturally occurring amphibian skin opioid peptide, has already demonstrated the presence of immunoreactive neurons in rat midbrain. Double immunostaining identified these neurons as a subpopulation of the mesencephalic dopaminergic neurons that were also tyrosine hydroxylase-immunopositive and calbindin- D28kD- negative, namely, the neurons predominantly affected in Parkinson disease. We followed the fate of these neurons after a monolateral injection of 6-hydroxy-dopamine into rat brain. Almost all the immunopositive neurons and their nigrostriatal, mesolimbic and mesocortical projections on the side ipsilateral to the lesion disappeared. Only a few scattered immunopositive neurons within the substantia nigra, pars compacta, and those of supramammillary nucleus remained unaffected. The consistent overlap of dopamine and this new molecule provides a further key to identifying the mammalian counterpart of these amphibian skin opioid peptides.
机译：对（D-Ala2）deltorphin-I（一种天然存在的两栖类皮肤阿片肽）的C末端四肽酰胺具有特异性的多克隆抗血清，已经证明在大鼠中脑中存在免疫反应性神经元。双重免疫染色将这些神经元鉴定为中脑多巴胺能神经元的亚群，中脑多巴胺能神经元也是酪氨酸羟化酶免疫阳性和calbindin-D28kD-阴性的，即主要受帕金森病影响的神经元。在将6-羟基-多巴胺单侧注射到大鼠脑中之后，我们追踪了这些神经元的命运。病灶同侧的几乎所有免疫阳性神经元及其黑质纹状体，中边缘和中皮层突起均消失。黑质，致密性腮腺和上乳头髓核中只有少数分散的免疫阳性神经元未受影响。多巴胺与这种新分子的一致重叠为鉴定这些两栖类皮肤阿片样肽的哺乳动物对应物提供了进一步的关键。

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