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首页> 外文期刊>European Journal of Histochemistry >The usefulness of c-Kit in the immunohistochemical assessment of melanocytic lesions
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The usefulness of c-Kit in the immunohistochemical assessment of melanocytic lesions

机译：c-Kit在黑色素细胞病变的免疫组织化学评估中的有用性

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C-Kit (CD117), the receptor for the stem cell factor, a growth factor for melanocyte migration and proliferation, has shown differential immunostaining in various benign and malignant melanocytic lesions. The purpose of this study is to compare c-Kit immunostaining in benign nevi and in primary and metastatic malignant melanomas, to determine whether c-Kit can aid in the differential diagnosis of these lesions. c-Kit immunostaining was performed in 60 cases of pigmented lesions, including 39 benign nevi (5 blue nevi, 5 intradermal nevi, 3 junctional nevi, 15 cases of primary compound nevus, 11 cases of Spitz nevus), 18 cases of primary malignant melanoma and 3 cases of metastatic melanoma. The vast majority of nevi and melanomas examined in this study were positive for c-Kit, with minimal differences between benign and malignant lesions. C-Kit cytoplasmatic immunoreactivity in the intraepidermal proliferating nevus cells, was detected in benign pigmented lesions as well as in malignant melanoma, increasing with the age of patients (P=0.007) in both groups. The patient’s age at presentation appeared to be the variable able to cluster benign and malignant pigmented lesions. The percentage of c-Kit positive intraepidermal nevus cells was better associated with age despite other variables (P=0.014). The intensity and percentage of c-Kit positivity in the proliferating nevus cells in the dermis was significantly increased in malignant melanocytic lesions (P=0.015 and P=0.008) compared to benign lesions (compound melanocytic nevi, Spitz nevi, intradermal nevi, blue nevi). Immunostaning for c-Kit in metastatic melanomas was negative. Interestingly in two cases of melanoma occurring on a pre-existent nevus, the melanoma tumor cells showed strong cytoplasmatic and membranous positivity for c-kit, in contrast with the absence of any immunoreactivity in pre-existent intradermal nevus cells. C-Kit does not appear to be a strong immunohistochemical marker for distinguishing melanoma from melanocytic nevi, if we consider c-Kit expression in intraepidermal proliferating cells. The c-Kit expression in proliferating melanocytes in the dermis could help in the differential diagnosis between a superficial spreading melanoma (with dermis invasion) and a compound nevus or an intradermal nevus. Finally, c-Kit could be a good diagnostic tool for distinguishing benign compound nevi from malignant melanocytic lesions with dermis invasion and to differentiate metastatic melanoma from primary melanoma.
机译：C-Kit（CD117）是干细胞因子（一种黑色素细胞迁移和增殖的生长因子）的受体，已在各种良性和恶性黑素细胞病变中显示出不同的免疫染色。这项研究的目的是比较良性痣，原发性和转移性恶性黑色素瘤中的c-Kit免疫染色，以确定c-Kit是否有助于这些病变的鉴别诊断。 c-Kit免疫染色在60例色素沉着病变中进行，包括39例良性痣（5例蓝色痣，5例皮内痣，3例交界性痣，15例原发性复合痣，11例Spitz痣），18例原发性恶性黑色素瘤3例转移性黑色素瘤。在这项研究中检查的绝大多数痣和黑色素瘤对c-Kit呈阳性，在良性和恶性病变之间差异最小。在良性色素性病变和恶性黑色素瘤中，在表皮内增生痣细胞中检测到C-Kit细胞质免疫反应性，两组均随着患者年龄的增加而增加（P = 0.007）。患者出现时的年龄似乎是能够使良性和恶性色素沉着病变聚集的变量。尽管有其他变量，但c-Kit阳性表皮内痣细胞的百分比与年龄相关性更好（P = 0.014）。与良性病变（复合性黑素细胞痣，斯皮茨痣，皮内痣，蓝色痣）相比，恶性黑素细胞病变（P = 0.015和P = 0.008）真皮中增殖的痣细胞中c-Kit阳性的强度和百分比显着增加。 ）。转移性黑色素瘤中对c-Kit的免疫测定为阴性。有趣的是，在先前存在的痣上发生的两个黑色素瘤病例中，黑色素瘤肿瘤细胞对c-kit表现出强的细胞质和膜阳性，与之相反，在既存的真皮内痣细胞中则没有任何免疫反应性。如果我们考虑表皮内增殖细胞中的c-kit表达，则c-kit似乎不是区分黑素瘤和黑素细胞痣的强有力的免疫组化标记。真皮中黑色素细胞增生中的c-Kit表达可能有助于鉴别浅表性黑色素瘤（真皮浸润）与复合痣或皮内痣。最后，c-Kit可能是一种很好的诊断工具，可将良性复合物痣与恶性黑色素细胞病变（真皮侵袭）区分开，并将转移性黑色素瘤与原发性黑色素瘤区分开。

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