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首页> 外文期刊>European Journal of Histochemistry >Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.)
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Histological and immunohistochemical investigation on ovarian development and plasma estradiol levels in the swordfish (Xiphias gladius L.)

机译：剑鱼卵巢发育和血浆雌二醇水平的组织学和免疫组化研究

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摘要

The paper reports a histological and immunohistochemical description of oocyte growth and ultrastructural aspects of zona radiata (ZR) formation as well as the relationship between plasma estradiol-17b (E2) levels and ovarian development in swordfish (Xiphias gladius L.) from the Mediterranean Sea. Ovaries were inactive during March to mid April; maturation occurred during late April to June and spawning in June and July. Zona radiata formation starts, as Pas positive material, in oocytes at the lipid stage. In this stage a deposit of electrondense material between oolemma and follicular cells appears. In the cortical alveoli stage and through the early vitellogenic stage, the deposition of a moderately electrondense material occurred on the inner side of the ZR. Finally, in late vitellogenic oocytes a third layer, made of microfibrillar material, appeared. The immunohistochemical analyses revealed that the initial internalisation of hepatic zona radiata proteins (Zrp) in the swordfish oocyte starts before the uptake of vitellogenin (Vtg) and that it is associated with the low previtellogenic E2 plasma levels, while a significant E2 increase in plasma is associated with the beginning of Vtg uptake. This would appear to confirm the hypothesis that the differential and sequential induction of zonagenesis and vitellogenesis may reflect a general feature of teleost oogenesis.
机译：该论文报道了来自地中海的剑鱼（Xiphias gladius L.）卵母细胞生长和放射带（ZR）形成的超微结构方面以及血浆雌二醇17b（E2）水平与卵巢发育之间的关系的组织学和免疫组化描述。 。卵巢在3月至4月中旬处于非活动状态;成熟发生在4月下旬至6月，并在6月和7月产卵。在脂质阶段，卵母细胞中作为Pas阳性物质的放射带形成开始。在这一阶段，出现电子致密物质在血肿和滤泡细胞之间的沉积。在皮质肺泡阶段和整个卵黄形成早期，中等电子密度物质的沉积发生在ZR的内侧。最后，在晚期卵黄卵母细胞中出现了由微纤维材料制成的第三层。免疫组织化学分析显示，箭鱼卵母细胞中的肝透明带辐射蛋白（Zrp）的初始内在化在卵黄蛋白原（Vtg）摄取之前开始，并且与低玻璃化前E2血浆水平有关，而血浆E2显着增加是与开始摄取Vtg有关。这似乎证实了这样的假设，即，造口作用和卵黄发生的差异和顺序诱导可能反映了硬骨鱼卵的一般特征。

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