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首页> 外文期刊>European Journal of Dentistry >Comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens
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Comparative evaluation of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, and a proton pump inhibitor against resistant root canal pathogens

机译：三种抗生素糊剂，氢氧化钙和质子泵抑制剂对耐药性根管病原体的抗菌效果比较评估

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Objective: The objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (TAP) and a proton pump inhibitor (PPI) (omeprazole) in combination with calcium hydroxide (CH) against Enterococcus faecalis and Candida albicans. Materials and Methods: E. faecalis and C. albicans were subcultured and inoculated at 37° overnight and were treated with different dilutions of TAP, 25 μg/ml (Group 1), CH (Group 2, control), CH 16 mg/ml + omeprazole 2 mg/ml (Group 3a) (CH 16 mg/ml + omeprazole 4 mg)/ml (Group 3b) for 24, 48, and 72 h in sterile uncoated 96-well microtiter plates. Minimum concentration at which the medicaments produced least optical density was determined using ELISA reader (ELx 808 BioTek Inc., USA) device set at optical density of 630 nm. Results were analyzed statistically by one-way analysis of variance followed by Tukey's multiple comparison tests. The significance level was set at 0.05. Results: Mean concentration (irrespective of time) for TAP at which mean minimum optical density was recorded at 1.25 μg/ml (1:20 dilution) and 25 μg/ml (0 dilution) against E. faecalis and C. albicans, respectively. Least optical density for CH plus PPI group was obtained 1.6 μg/ml (1:10 dilution) and 16 μg/ml (0 dilution) for E. faecalis and C. albicans, respectively. However, CH alone showed a weaker antimicrobial action against either of the strains even at full concentration. Conclusions: PPI enhanced the antibacterial efficacy of CH against E. faecalis and C. albicans. However, TAP showed the best antibacterial property followed by CH plus PPIs against both the selected strains.
机译：目的：本研究的目的是比较三联抗生素糊剂（TAP）和质子泵抑制剂（PPI）（奥美拉唑）联合氢氧化钙（CH）对粪肠球菌和白色念珠菌的抗菌作用。材料和方法：将粪肠球菌和白色念珠菌继代培养并在37°接种过夜，并用不同稀释度的TAP，25μg/ ml（第1组），CH（第2组，对照），CH 16 mg / ml进行处理+无菌奥美拉唑2 mg / ml（组3a）（CH 16 mg / ml +奥美拉唑4 mg）/ ml（组3b）在无菌无涂层96孔微量滴定板中放置24、48和72小时。使用设置为630 nm的光密度的ELISA阅读器（ELx 808 BioTek Inc.，美国）设备确定药物产生最小光密度的最小浓度。通过单向方差分析和随后的Tukey多重比较检验对结果进行统计分析。显着性水平设定为0.05。结果：TAP的平均浓度（与时间无关），分别记录了对屎肠球菌和白色念珠菌的平均最小光密度为1.25μg/ ml（1:20稀释）和25μg/ ml（0稀释）。 CH加PPI组的最低光密度分别为粪肠球菌和白色念珠菌1.6μg/ ml（1:10稀释）和16μg/ ml（0稀释）。然而，即使在全浓度下，单独的CH对两种菌株都显示出较弱的抗菌作用。结论：PPI增强了CH对粪肠球菌和白色念珠菌的抗菌作用。但是，TAP表现出最佳的抗菌性能，其次是CH加PPI对两种所选菌株。

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