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Study on the effects of parecoxib on hypothalamus orexin neuron of cerebral infarction rats

机译：帕瑞昔布对脑梗死大鼠下丘脑食欲素神经元影响的研究

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OBJECTIVE: To explore the effect of parecoxib on cerebral infarction in rats and the regulatory mechanism on hypothalamus orexin neurons (orexin) and protein expression. MATERIALS AND METHODS: 60 SD male rats were randomly divided into sham operation group, model group and treatment group (20 rats in each group). Cerebral infarction model was established by modified Longa method. Rats in the treatment group were given parecoxib (2.5 mg kg-1) in tail by intravenous injection, while both the sham operation group and the model group were given the equal volume of sterile PBS solution in the tail vein. Continuous intervention of 72h was carried out in the three groups. Immunofluorescence staining and Western blot were used to detect the expression of orexin neurons and orexin protein in the hypothalamus of rats, respectively. RESULTS: Immunofluorescence staining showed that the number of orexin positive cells in the model group was significantly less than that in the sham-operated group (p < 0.01). After treatment intervention, the number of orexin positive cells in the hypothalamus was significantly increased compared to that in model group (p < 0.01). Western blot analysis showed that compared with sham operation group, the expression of orexin in the hypothalamus of model group was significantly decreased (p < 0.01), whereas the expression of orexin protein was significantly elevated after parecoxib intervention (p < 0.01). CONCLUSIONS: Parecoxib plays a therapeutic effect on cerebral infarction by up-regulating the orexin neuron.
机译：目的：探讨帕瑞昔布对大鼠脑梗死的作用及其对下丘脑食欲素神经元（orexin）和蛋白质表达的调控机制。材料与方法：将60只SD雄性大鼠随机分为假手术组，模型组和治疗组，每组20只。采用改良的Longa方法建立脑梗死模型。治疗组大鼠尾静脉注射帕瑞昔布（2.5 mg kg-1），假手术组和模型组均在尾静脉中给予等体积的无菌PBS溶液。三组均进行了连续72h干预。免疫荧光染色和蛋白质印迹法分别检测大鼠下丘脑中orexin神经元和orexin蛋白的表达。结果：免疫荧光染色显示，模型组的食欲素阳性细胞数量明显少于假手术组（p <0.01）。在治疗干预后，下丘脑中食欲素阳性细胞的数量与模型组相比明显增加（p <0.01）。 Western blot分析显示，与假手术组相比，模型组下丘脑中orexin的表达明显降低（p <0.01），而帕瑞昔布干预后orexin蛋白的表达明显升高（p <0.01）。结论：帕瑞昔布通过上调食欲素神经元对脑梗死具有治疗作用。

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