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首页> 外文期刊>Neuroscience Research: The Official Journal of the Japan Neuroscience Society >Morphological study of orexin neurons in the hypothalamus of the Long-Evans rat, with special reference to co-expression of orexin and NADPH-diaphorase or nitric oxide synthase activities.
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Morphological study of orexin neurons in the hypothalamus of the Long-Evans rat, with special reference to co-expression of orexin and NADPH-diaphorase or nitric oxide synthase activities.

机译：Long-Evans大鼠下丘脑中食欲素神经元的形态学研究，特别涉及食欲素和NADPH-心肌黄递酶或一氧化氮合酶活性的共表达。

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Orexins, novel neuropeptides, are exclusively localized in the hypothalamus and implicated in the regulation of a variety of activities, including food intake and energy balance. Nitric oxide (NO), an unconventional neurotransmitter, is widely present in numerous brain regions including the hypothalamus, and has similar physiological roles to those of the orexins. The present study was undertaken to examine the distribution of orexin neurons and the presence of neuronal nitric oxide synthase (nNOS) in the orexin neurons to clarify whether NO interacts with the orexins in the neuronal regulation activities in the Long-Evans rat. We used two double-labeling methods: nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry in combination with orexin immunohistochemistry, and double-labeling fluorescent immunohistochemistry for orexin and nNOS. The majority of the orexin immunoreactive neurons were localized mainly in the areas of the dorsomedial hypothalamic nucleus (DMN), the dorsal part of the perifornical nucleus (PEF) and lateral hypothalamic area. The orexin immunoreactive cell bodies were medium in size, and triangular, round, elliptic, and fusiform in shape. The sizes and shapes of orexin neurons in the different parts were similar. Cell bodies coexpressing the orexin and nNOS or NADPH-d were present in the areas of the DMN and the PEF, and the nerve fibers containing orexin and nNOS were distributed in the DMN and PEF, arcuate nucleus (ARN) and ventromedial hypothalamic nucleus (VMH). These results provide morphological evidence that there exists a population of nNOS- or NADPH-d-/orexin-coexpressing neurons in the orexinergic cell group in the hypothalamus, and taken together with previous findings, suggest that NO may play a role in the mechanisms by which orexin neurons regulate food intake and energy balance.
机译：新型神经肽食欲素专门位于下丘脑，并参与多种活动的调节，包括食物摄入和能量平衡。一氧化氮（NO）是一种非常规的神经递质，广泛存在于包括下丘脑在内的许多大脑区域中，并且具有与食欲素相似的生理作用。本研究旨在检查orexin神经元中orexin神经元的分布和神经元一氧化氮合酶（nNOS）的存在，以阐明Long-Evans大鼠在神经元调节活动中NO是否与orexins相互作用。我们使用了两种双重标记方法：烟酰胺腺嘌呤二核苷酸磷酸心肌黄酶（NADPH-d）的组织化学结合orexin免疫组织化学，以及针对orexin和nNOS的双重标记荧光免疫组织化学。大部分的orexin免疫反应性神经元主要位于背部丘脑下丘脑核（DMN），椎弓根周围核的背面部分（PEF）和下丘脑外侧区域。食欲素免疫反应性细胞体大小中等，形状为三角形，圆形，椭圆形和梭形。食欲素神经元在不同部位的大小和形状相似。在DMN和PEF区域中存在共表达orexin和nNOS或NADPH-d的细胞体，而含有orexin和nNOS的神经纤维分布在DMN和PEF，弓形核（ARN）和下丘脑下丘脑核（VMH）中）。这些结果提供了形态学证据，表明下丘脑的食欲能细胞群中存在大量的表达nNOS或NADPH-d / orexin的神经元，并与先前的发现结合起来表明，NO可能通过以下途径在机制中起作用：哪些食欲素神经元调节食物摄入和能量平衡。

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