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首页> 外文期刊>European Journal of Preventive Medicine >Effects of Vitamin D_3 on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Biofield Energy Healing Treatment
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Effects of Vitamin D_3 on the Proliferation and Mineralization of Human Osteoblast-Like Cells: Implications of Biofield Energy Healing Treatment

机译：维生素D_3对人类成骨细胞样细胞增殖和矿化的影响：生物领域能量治疗的意义。

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Silently progressing metabolic bone disorders are widely increased that affects the quality of life as one of the key health issues today. The aim of the study is to evaluate the potential of Consciousness Energy Healing based vitamin D_3 and DMEM medium on bone health parameters. Vitamin D_3 and DMEM medium as test items (TI), were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Thomas Charles Slade and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization as bone health biomarkers were studied. The cell viability (>84%) using MTT assay showed that the test items were found to be safe. ALP level was significantly increased by 218.6% (100 μg/mL), 263.9% (10 μg/mL), and 135.7% (100 μg/mL) in the UT-DMEM+BT-TI, BTDMEM+ UT-TI, and BT-DMEM+BT-TI groups, respectively. Collagen content was significantly increased by 147.6%, 153.1%, and 240.8% at 10, 50, and 100 μg/mL, respectively in the UT-DMEM+BT-TI group, while 150.8% and 212.9% at 50 and 100 μg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 104.6% and 185.9% in UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively at 10 μg/mL, while 119%, 42.6%, and 102.3% at 0.1, 1, and 10 μg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Overall, the Biofield Energy Treated vitamin D_3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D_3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, Paget's disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures.
机译：无声进展的代谢性骨疾病已广泛增加，这已成为当今生活中的关键健康问题之一，影响了生活质量。该研究的目的是评估基于意识能量治疗的维生素D_3和DMEM培养基对骨骼健康参数的潜力。维生素D_3和DMEM培养基作为测试项目（TI），分为两部分。每个样品都接受了Thomas Charles Slade的“意识能量治疗”治疗，这些样品被标记为经过Biofield能量治疗（BT）的样品，而每个样品的其他部分则称为未处理的测试项目（UT）。研究了碱性磷酸酶（ALP）的活性，胶原蛋白水平和骨矿化作为骨骼健康的生物标志物。使用MTT分析的细胞活力（> 84％）表明测试项目是安全的。在UT-DMEM + BT-TI，BTDMEM + UT-TI和BT中，ALP水平显着提高了218.6％（100μg/ mL），263.9％（10μg/ mL）和135.7％（100μg/ mL） -DMEM + BT-TI组。在UT-DMEM + BT-TI组中，分别在10、50和100μg/ mL下胶原蛋白含量分别显着增加了147.6％，153.1％和240.8％，而在50和100μg/ mL下胶原蛋白含量分别增加了150.8％和212.9％。与未处理组相比，BT-DMEM + BT-TI组分别为50 mL。此外，UT-DMEM + BT-TI和BT-DMEM + BT-TI组的骨矿化百分比分别以10μg/ mL显着增加104.6％和185.9％，而119％，42.6％和102.3与未处理组相比，BT-DMEM + UT-TI组分别以0.1、1和10μg/ mL的浓度％。总体而言，在许多骨骼疾病中，经过Biofield能量处理的维生素D_3和DMEM在控制成骨细胞功能，改善骨骼矿化和钙吸收方面将发挥重要作用。此外，骨骼健康参数（例如胶原蛋白，钙和ALP）得到显着改善，可以用作改善骨骼健康的补充。根据出色的结果，可以认为经过Biofield能量治疗的维生素D_3可以作为强大的替代饮食来源和补品，与各种与骨骼相关的疾病（包括低骨密度和骨质疏松症，佩吉特氏骨病，病，畸形骨骼，骨软化症）作斗争。 ，骨骼和/或关节疼痛，骨折频率增加，骨瘤，荷尔蒙失调，压力，衰老，骨质流失和骨折。

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