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首页> 外文期刊>European review for medical and pharmacological sciences. >Sivelestat sodium hydrate attenuates acute lung injury by decreasing systemic inflammation in a rat model of severe burns
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Sivelestat sodium hydrate attenuates acute lung injury by decreasing systemic inflammation in a rat model of severe burns

机译：Sivelestat水合钠通过减轻严重烧伤大鼠的全身炎症反应减轻急性肺损伤

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OBJECTIVE: Patients with severe burns often develop acute lung injury (ALI), systemic inflammatory response syndrome (SIRS) often complicates with ALI. Sivelestat sodium hydrate is an effective drug against ALI. However, the mechanisms of this beneficial effect are still poorly understood. In the current study, we evaluate the effects of sivelestat sodium hydrate on systemic and local inflammatory parameters (neutrophil elastase [NE], interleukin [IL]-8, matrix metalloproteinase [MMP] 2 and 9) in a rat model of severe burns and ALI. And to analyze the correlations between expression of NE and IL-8 and acute lung injury. MATERIALS AND METHODS: 48 Sprague-Dawley (SD) rats were divided into 3 groups: normal control group, severe burns injury group and severe burns treated with sivelestat sodium hydrate group (SSI). The lung water content and PaO2 were detected in each group. Pathological manifestations in each group were observed for pathology scoring in SD rats with acute lung injury. ELISA was used for detecting expression of NE and IL-8 in serum and BAL specimens of SD rats in each group. RT-PCR was used to detect mRNA expression of NE and IL-8 in lung tissues of each group. Western blotting was used for detecting protein expression of MMP-2 and MMP-9 in lung tissues of each group. SPSS 18.0 was used for statistical analysis. RESULTS: The PaO2 was significantly increased after sivelestat sodium hydrate intravenous injection. Pathological score and water content of lung tissue were significantly decreased in SSI group compared with severe burns injury group, slightly higher than that normal control group. NE and IL-8 levels significantly decreased in serum, BAL and lung tissue specimens after sivelestat sodium hydrate intravenous injection; Expression of MMP-2 and MMP-9 were significantly up-regulated in severe burns group and showed no significantly changed after sivelestat sodium hydrate intravenous injection. CONCLUSIONS: In a rat model of severe burns and ALI, administration of sivelestat sodium hydrate improved symptoms of ALI and significantly decreased inflammatory parameters NE and IL-8.
机译：目的：严重烧伤的患者通常会发展为急性肺损伤（ALI），全身性炎症反应综合征（SIRS）通常会并发ALI。 Sivelestat水合钠是抗ALI的有效药物。但是，这种有益作用的机制仍知之甚少。在当前的研究中，我们评估在严重烧伤和烧伤大鼠模型中，西乐司他钠水合物对全身和局部炎症参数（中性粒细胞弹性蛋白酶[NE]，白介素[IL] -8，基质金属蛋白酶[MMP] 2和9）的影响。 ALI。并分析NE和IL-8的表达与急性肺损伤的相关性。材料与方法：将48只SD大鼠分为3组：正常对照组，重度烧伤损伤组和Silelestat水合钠治疗严重烧伤组。在每组中检测肺水含量和PaO 2。观察每组在急性肺损伤SD大鼠中的病理学评分以进行病理评分。 ELISA法检测各组SD大鼠血清和BAL标本中NE和IL-8的表达。 RT-PCR检测各组肺组织NE和IL-8的mRNA表达。 Western blotting检测每组肺组织中MMP-2和MMP-9的蛋白表达。使用SPSS 18.0进行统计分析。结果：塞来司他钠水合静脉注射后，PaO2明显升高。与严重烧伤相比，SSI组肺组织病理评分和含水量明显降低，略高于正常对照组。盐酸西乐司他钠静脉注射后血清，BAL和肺组织标本中NE和IL-8水平明显降低;重度烧伤组MMP-2和MMP-9的表达明显上调，而西乐司他钠水合静脉注射后无明显变化。结论：在严重烧伤和ALI的大鼠模型中，给予ilelestat钠水合物可改善ALI症状，并显着降低炎症参数NE和IL-8。

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