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首页> 外文期刊>Environments >Establishment of an Aseptic Culture System and Analysis of the Effective Growth Conditions for Eleocharis acicularis Ramets for Use in Phytoremediation
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Establishment of an Aseptic Culture System and Analysis of the Effective Growth Conditions for Eleocharis acicularis Ramets for Use in Phytoremediation

机译：用于植物修复的针状Eleocharis acicularis分株的无菌培养系统的建立和有效生长条件的分析

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Eleocharis acicularis , an aquatic macrophyte of the Cyperaceae family, has been shown to accumulate multiple heavy metals and has great potential for use in the phytoremediation of contaminated soil and water. To investigate the mechanism of accumulation of heavy metals in E. acicularis while excluding biotic and abiotic environmental effects and to acquire homogenous and sufficient populations of E. acicularis , we established an aseptic culture system and analyzed the applicability of this species for phytoremediation. Young ramet bases and stolon tips of E. acicularis grown in the field were sterilized, cultured, and established in an aseptic culture system, and the effective growth conditions of isolated ramets were determined. Isolated ramets grew remarkably well in a medium of pH 4.8 to 5.7 with 0.25 mg/L kinetin as a plant hormone. Furthermore, capacity for the accumulation of heavy metals was examined using E. acicularis subcultured with or without Si. Aseptically cultured E. acicularis showed a sufficient capacity for Cs and Zn accumulation and exceeded the criteria for hyperaccumulator plants in accumulating Pb, Cd, and In regardless of the addition or not of Si during its subculture. The aseptic culture of E. acicularis enhances its capacity for the accumulation of heavy metals and its applicability for phytoremediation.
机译：莎草科的水生大型植物针叶树Eleocharis acicularis已显示出积累了多种重金属，在被污染的土壤和水中进行植物修复方面具有巨大的潜力。为了研究针状大肠杆菌中重金属的积累机制，同时排除生物和非生物环境影响，并获取均匀和足够的针状大肠杆菌种群，我们建立了无菌培养系统，并分析了该物种在植物修复中的适用性。在无菌培养系统中对田间生长的大肠埃希氏菌的幼小分株基础和茎尖端进行灭菌，培养和建立，并确定分离分株的有效生长条件。分离的分株在0.25 mg / L激动素作为植物激素的pH 4.8至5.7培养基中生长良好。此外，使用在有或没有Si的情况下亚培养的针状大肠杆菌（E. acicularis）检查了重金属积累的能力。无菌培养的针状大肠杆菌显示出足够的Cs和Zn积累能力，并且无论其继代培养过程中是否添加了Si，都超过了超积累植物积累Pb，Cd和In的标准。针状肠杆菌的无菌培养提高了其重金属积累的能力和其植物修复的适用性。

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