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Identification of Whey Acidic Protein (WAP) in Dog Milk

机译：犬乳中乳清酸性蛋白（WAP）的鉴定

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Whey acidic protein (WAP) has been identified as a major whey protein in milk of a wide range of species and reportedly plays important roles in regulating the proliferation of mammary epithelial cells. However, in some species including humans, WAP is not synthesized in the mammary gland. The presence of WAP in carnivore species has not been reported. We searched the National Center for Biotechnology Information (NCBI) database for the dog WAP gene and tried biochemically to identify WAP in dog milk. The nucleotide sequence of the examined dog genomic DNA was completely identical to that in the NCBI database and showed that the dog WAP gene, like other known functional WAP genes, has four exons. Biochemical analysis of milk protein by reverse-phase HPLC and Western blotting demonstrated the presence of WAP in dog milk.
机译：乳清酸性蛋白（WAP）已被确定为多种物种的牛奶中的主要乳清蛋白，据报道在调节乳腺上皮细胞的增殖中起重要作用。但是，在包括人类在内的某些物种中，WAP不是在乳腺中合成的。食肉动物物种中WAP的存在尚未见报道。我们搜索了国家生物技术信息中心（NCBI）数据库中的狗WAP基因，并尝试进行了生化鉴定以鉴定狗乳中的WAP。检查的狗基因组DNA的核苷酸序列与NCBI数据库中的核苷酸序列完全相同，表明狗WAP基因与其他已知的功能性WAP基因一样具有四个外显子。通过反相HPLC和Western印迹对牛奶蛋白进行生化分析表明，狗奶中存在WAP。

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