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首页> 外文期刊>Experimental Animals >Effect of Catha edulis (khat) on pancreatic functions in streptozotocin-induced diabetes in male Sprague-Dawley rats
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Effect of Catha edulis (khat) on pancreatic functions in streptozotocin-induced diabetes in male Sprague-Dawley rats

机译：食用茶对链脲佐菌素诱发的雄性Sprague-Dawley大鼠糖尿病胰腺功能的影响

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People consume Catha edulis (khat) for its euphoric effect, and type 1 diabetics have claimed that khat could reduce elevated levels of blood sugar. However, khat has been suggested to provoke diabetes mellitus through destruction of pancreatic β-cells. This study investigated the effect of an ethanolic khat extract on pancreatic functions in type 1 diabetes (T1DM)-induced male Sprague-Dawley rats and to assess its in vitro cytotoxicity in rat pancreatic β-cells (RIN-14B). T1DM was induced in a total of 20 rats with a single intraperitoneal injection of 75 mg/kg of streptozotocin. The rats were distributed into four groups (n=5): the diabetic control, 8 IU insulin-treated, 200 mg/kg khat-treated, and 400 mg/kg khat-treated groups. Another 5 rats were included as a nondiabetic control. Body weight, fasting blood sugar, and caloric intake were recorded weekly. Four weeks after treatment, the rats were sacrificed, and blood was collected for insulin, lipid profile, total protein, amylase, and lipase analysis, while pancreases were harvested for histopathology. In vitro , khat exerted moderate cytotoxicity against RIN-14B cells after 24 and 48 h but demonstrated greater inhibition against RIN-14B cells after 72 h. Neither 200 mg/kg nor 400 mg/kg of khat produced any significant reduction in blood sugar; however, 200 mg/kg khat extract provoked more destruction of pancreatic β-cells as compared with the diabetic control. Ultimately, neither 200 mg/kg nor 400 mg/kg of khat extract could produce a hypoglycemic effect in T1DM-induced rats. However, 200 mg/kg of khat caused greater destruction of pancreatic β-cells, implying that khat may cause a direct cytotoxic effect on pancreatic β-cells in vitro .
机译：人们食用其具有欣快感的Catha edulis（khat），1型糖尿病患者声称卡塔叶可以降低血糖升高的水平。但是，已经有人提出通过破坏胰岛β细胞来激发糖尿病。这项研究调查了乙醇卡塔叶提取物对1型糖尿病（T1DM）诱导的雄性Sprague-Dawley大鼠胰腺功能的影响，并评估了其对大鼠胰腺β细胞（RIN-14B）的体外细胞毒性。一次腹膜内注射75 mg / kg链脲佐菌素可在总共20只大鼠中诱导T1DM。将大鼠分为四组（n = 5）：糖尿病对照组，8 IU胰岛素治疗组，200 mg / kg卡特治疗组和400 mg / kg卡特治疗组。包括另外5只大鼠作为非糖尿病对照。每周记录体重，空腹血糖和热量摄入。治疗后四周，处死大鼠，收集血液用于胰岛素，脂质分布，总蛋白，淀粉酶和脂肪酶分析，同时收集胰腺进行组织病理学检查。在体外，卡塔叶在24和48小时后对RIN-14B细胞产生中等的细胞毒性，但在72小时后表现出对RIN-14B细胞的更大抑制作用。 200 mg / kg或400 mg / kg的卡塔叶既没有产生任何血糖的明显降低;然而，与糖尿病对照组相比，200 mg / kg的卡塔叶提取物引起了胰腺β细胞的更多破坏。最终，200 mg / kg或400 mg / kg的卡塔叶提取物都不会对T1DM诱导的大鼠产生降血糖作用。然而，200 mg / kg的卡塔叶会引起胰腺β细胞的更大破坏，这意味着卡塔叶可能在体外对胰腺β细胞产生直接的细胞毒性作用。

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