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首页> 外文期刊>Eukaryotic cell >The Phosducin-Like Protein PhnA Is Required for Gβγ-Mediated Signaling for Vegetative Growth, Developmental Control, and Toxin Biosynthesis in Aspergillus nidulans
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The Phosducin-Like Protein PhnA Is Required for Gβγ-Mediated Signaling for Vegetative Growth, Developmental Control, and Toxin Biosynthesis in Aspergillus nidulans

机译：Gβγ介导的信号传导需要信息素类蛋白PhnA，以促进构巢曲霉的营养生长，发育控制和毒素生物合成。

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Phosducin or phosducin-like protein (PhLP) is a positive regulator of Gβγ activity. The Gβ (SfaD) and Gγ (GpgA) subunits function in vegetative growth and developmental control in the model filamentous fungus Aspergillus nidulans. To better understand the nature of Gβγ-mediated signaling, phnA, encoding an A. nidulans PhLP, has been studied. Deletion of phnA resulted in phenotypes almost identical to those caused by deletion of sfaD, i.e., reduced biomass, asexual sporulation in liquid submerged culture, and defective fruiting body formation, suggesting that PhnA is necessary for Gβ function. The requirement for the RGS protein FlbA in asexual sporulation could be bypassed by the ΔphnA mutation, indicating that PhnA functions in FlbA-controlled vegetative growth signaling, primarily mediated by the heterotrimeric G protein composed of FadA (Gα), SfaD, and GpgA. However, whereas deletion of fadA restored both asexual sporulation and the production of sterigmatocystin (ST), deletion of sfaD, gpgA, or phnA failed to restore ST production in the ΔflbA mutant. Further studies revealed that SfaD, GpgA, and PhnA are necessary for the expression of aflR, encoding the transcriptional activator for the ST biosynthetic genes, and subsequent ST biosynthesis. Overexpression of aflR bypassed the need for SfaD in ST production, indicating that the results of SfaD-mediated signaling may include transcriptional activation of aflR. Potential differential roles of FadA, Gβγ, and FlbA in controlling ST biosynthesis are further discussed.
机译：Phosducin或Phsducin样蛋白（PhLP）是Gβγ活性的正调节剂。 Gβ（SfaD）和Gγ（GpgA）亚基在模型丝状真菌 Nidulans 的营养生长和发育控制中起作用。为了更好地理解Gβγ介导的信号传导的本质， phnA 编码 A。 nidulans PhLP已被研究。删除 phnA 导致的表型几乎与删除 sfaD 引起的表型相同，即生物量减少，液体淹没培养中的无性孢子形成和子实体形成缺陷，这表明PhnA是Gβ功能所必需的。 Δ phnA 突变可绕过无性孢子中RGS蛋白FlbA的需求，这表明PhnA在FlbA控制的营养生长信号中发挥功能，主要由由FadA（Gα ），SfaD和GpgA。但是，删除 fadA 既可以恢复无性孢子形成，又可以恢复葡萄球菌毒素（ST）的产生，而删除 sfaD ， gpgA 或 phnA 无法恢复Δ flbA 突变体的ST产生。进一步的研究表明，SfaD，GpgA和PhnA对于表达 aflR ，编码ST生物合成基因的转录激活因子以及随后的ST生物合成是必需的。 aflR 的过表达绕过了ST生产中对SfaD的需求，这表明SfaD介导的信号转导的结果可能包括 aflR 的转录激活。进一步讨论了FadA，Gβγ和FlbA在控制ST生物合成中的潜在差异作用。

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