...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Eukaryotic cell >Dispersed Mutations in Histone H3 That Affect Transcriptional Repression and Chromatin Structure of the CHA1 Promoter in Saccharomyces cerevisiae
【24h】

Dispersed Mutations in Histone H3 That Affect Transcriptional Repression and Chromatin Structure of the CHA1 Promoter in Saccharomyces cerevisiae

机译：组蛋白H3中的分散突变影响酿酒酵母CHA1启动子的转录抑制和染色质结构。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The histone H3 amino terminus, but not that of H4, is required to prevent the constitutively bound activator Cha4 from remodeling chromatin and activating transcription at the CHA1 gene in Saccharomyces cerevisiae. Here we show that neither the modifiable lysine residues nor any specific region of the H3 tail is required for repression of CHA1. We then screened for histone H3 mutations that cause derepression of the uninduced CHA1 promoter and identified six mutants, three of which are also temperature-sensitive mutants and four of which exhibit a sin? phenotype. Histone mutant levels were similar to that of wild-type H3, and the mutations did not cause gross alterations in nucleosome structure. One specific and strongly derepressing mutation, H3 A111G, was examined in depth and found to cause a constitutively active chromatin configuration at the uninduced CHA1 promoter as well as at the ADH2 promoter. Transcriptional derepression and altered chromatin structure of the CHA1 promoter depend on the activator Cha4. These results indicate that modest perturbations in distinct regions of the nucleosome can substantially affect the repressive function of chromatin, allowing activation in the absence of a normal inducing signal (at CHA1) or of Swi/Snf (resulting in a sin? phenotype).
机译：需要组蛋白H3氨基端而非H4端基，以防止组成型结合的活化剂Cha4重塑染色质并激活 Saccharomyces cerevisiae 中 CHA1 基因的转录。在这里，我们证明抑制 CHA1 不需要修饰的赖氨酸残基或H3尾部的任何特定区域。然后，我们筛选了导致未诱导的 CHA1 启动子抑制的组蛋白H3突变，并鉴定了六个突变体，其中三个也是温度敏感突变体，其中四个表现出 sin ？表型。组蛋白突变体水平与野生型H3相似，并且该突变不会引起核小体结构的总体改变。深入研究了一种特异性且强烈抑制抑郁的突变体H3 A111G，发现其在未诱导的 CHA1 启动子和 ADH2 启动子上引起了组成型活性染色质构型。 CHA1 启动子的转录抑制和染色质结构改变取决于活化剂Cha4。这些结果表明，核小体不同区域的适度扰动会实质上影响染色质的抑制功能，从而在没有正常诱导信号（ CHA1 ）或Swi / Snf（导致 sin ？表型）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号