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首页> 外文期刊>Eukaryotic cell >Candida albicans Ferric Reductases Are Differentially Regulated in Response to Distinct Forms of Iron Limitation by the Rim101 and CBF Transcription Factors
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Candida albicans Ferric Reductases Are Differentially Regulated in Response to Distinct Forms of Iron Limitation by the Rim101 and CBF Transcription Factors

机译：白色念珠菌铁还原酶受Rim101和CBF转录因子对不同形式铁限制的响应的差异调节

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Iron is an essential nutrient that is severely limited in the mammalian host. Candida albicans encodes a family of 15 putative ferric reductases, which are required for iron acquisition and utilization. Despite the central role of ferric reductases in iron acquisition and mobilization, relatively little is known about the regulatory networks that govern ferric reductase gene expression in C. albicans. Here we have demonstrated the differential regulation of two ferric reductases, FRE2 and FRP1, in response to distinct iron-limited environments. FRE2 and FRP1 are both induced in alkaline-pH environments directly by the Rim101 transcription factor. However, FRP1 but not FRE2 is also induced by iron chelation. We have identified a CCAAT motif as the critical regulatory sequence for chelator-mediated induction and have found that the CCAAT binding factor (CBF) is essential for FRP1 expression in iron-limited environments. We found that a hap5Δ/hap5Δ mutant, which disrupts the core DNA binding activity of CBF, is unable to grow under iron-limited conditions. C. albicans encodes three CBF-dependent transcription factors, and we identified the Hap43 protein as the CBF-dependent transcription factor required for iron-limited responses. These studies provide key insights into the regulation of ferric reductase gene expression in the fungal pathogen C. albicans.
机译：铁是一种必需营养素，在哺乳动物宿主中受到严格限制。 白色念珠菌编码15种推定的铁还原酶家族，它们是铁的获取和利用所必需的。尽管铁还原酶在铁的获取和动员中起着核心作用，但对于控制 C中铁还原酶基因表达的调控网络知之甚少。白色的。在这里，我们展示了两种铁还原酶 FRE2 和 FRP1 的差异调节，以响应不同的铁限制环境。在碱性pH环境中，Rim101转录因子直接诱导 FRE2 和 FRP1 。但是，铁螯合也诱导了 FRP1 而不是 FRE2 。我们已经鉴定出CCAAT基序是螯合剂介导的诱导的关键调控序列，并且发现CCAAT结合因子（CBF）对于铁限制环境中的 FRP1 表达至关重要。我们发现 hap5 Δ/ hap5 Δ突变体破坏了CBF的核心DNA结合活性，在铁受限的条件下无法生长。 C。 albicans 编码三个CBF依赖性转录因子，我们确定Hap43蛋白是铁限制反应所需的CBF依赖性转录因子。这些研究为真菌病原体 C中铁还原酶基因表达的调控提供了重要见识。白色的。

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