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首页> 外文期刊>Eukaryotic cell >Identification of the Putative Protein Phosphatase Gene PTC1 as a Virulence-Related Gene Using a Silkworm Model of Candida albicans Infection
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Identification of the Putative Protein Phosphatase Gene PTC1 as a Virulence-Related Gene Using a Silkworm Model of Candida albicans Infection

机译：使用白色念珠菌感染的家蚕模型鉴定推定的蛋白磷酸酶基因PTC1作为毒力相关基因。

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Protein phosphatases are critical for the regulation of many cellular processes. Null mutants of 21 putative protein phosphatases of Candida albicans were constructed by consecutive allele replacement using the URA3 and ARG4 marker genes. A simple silkworm model of C. albicans infection was used to screen the panel of mutants. Four null mutant (cmp1Δ, yvh1Δ, sit4Δ, and ptc1Δ) strains showed attenuated virulence in the silkworm model relative to that of control and parental strains. Three of the mutants, the cmp1Δ, yvh1Δ, and sit4Δ mutants, had previously been identified as affecting virulence in a conventional mouse model, indicating the validity of the silkworm model screen. Disruption of the putative protein phosphatase gene PTC1 of C. albicans, which has 52% identity to the Saccharomyces cerevisiae type 2C protein phosphatase PTC1, significantly reduced virulence in the silkworm model. The mutant was also avirulent in a mouse model of disseminated candidiasis. Reintroducing either of the C. albicans PTC1 alleles into the disruptant strain, using a cassette containing either allele under the control of a constitutive ACT1 promoter, restored virulence in both infection models. Characterization of ptc1Δ revealed other phenotypic traits, including reduced hyphal growth in vitro and in vivo, and reduced extracellular proteolytic activity. We conclude that PTC1 may contribute to pathogenicity in C. albicans.
机译：蛋白质磷酸酶对于调节许多细胞过程至关重要。通过使用 URA3 和 ARG4 标记基因连续等位基因置换，构建了白色念珠菌的21种假定蛋白磷酸酶的空突变体。 C的简单蚕模型。用白色念珠菌感染筛选突变体。四个无效突变株（ cmp1 Δ， yvh1 Δ， sit4 Δ和 ptc1 Δ）菌株显示了弱毒力相对于对照和亲本菌株的蚕模型。在常规小鼠模型中，先前已经确定了三个突变体，分别是 cmp1 Δ， yvh1 Δ和 sit4 Δ突变体。 ，表明家蚕模型屏幕的有效性。 C的假定蛋白磷酸酶基因 PTC1 的破坏。与酿酒酵母（Saccharomyces cerevisiae）2em型2C蛋白磷酸酶 PTC1 具有52％的同一性的白色念珠菌，可显着降低家蚕模型中的毒力。该突变体在传播的念珠菌病小鼠模型中也无毒。重新引入两个 C。将白色念珠菌PTC1 等位基因导入破坏菌株，使用包含任一等位基因的盒在组成型 ACT1 启动子的控制下，在两种感染模型中均恢复了毒力。 ptc1 Δ的表征揭示了其他表型性状，包括体外和体内菌丝生长减少以及细胞外蛋白水解活性降低。我们得出结论， PTC1 可能与 C的致病性有关。白色的。

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