Molecular Cloning and Expression of Canine Prolactin Gene

Osamu HASHIMOTO; Tomohiro YONEZAWA; Yuka SUGIYAMA; Mitsumori KAWAMINAMI; Yoshihisa HASEGAWA

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Molecular Cloning and Expression of Canine Prolactin Gene

机译：犬催乳素基因的分子克隆与表达

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We isolated canine prolactin cDNA from a dog pituitary cDNA library. The 930-bp nucleotide sequence covered the entire open reading frame encoding the putative 229 amino acids. It was located in chromosome 35, and had five exons. The amino acid sequence was highly homologous to the feline and porcine sequences. To generate recombinant canine prolactin, plasmids for full-length canine prolactin were constructed and transfected into the mammalian HEK293 cell line. The recombinant prolactin was secreted into the medium as an N-linked glycosylated (31 kDa) or non-glycosylated (27 kDa) protein. Western blotting revealed both of these bands were canine pituitary protein extracts.

机译：我们从狗垂体cDNA文库中分离出犬催乳素cDNA。 930 bp的核苷酸序列覆盖了编码假定的229个氨基酸的整个开放阅读框。它位于第35号染色体上，有五个外显子。氨基酸序列与猫和猪序列高度同源。为了产生重组犬催乳素，构建了全长犬催乳素的质粒，并将其转染到哺乳动物HEK293细胞系中。重组催乳素以N-联糖基化（31 kDa）或非糖基化（27 kDa）蛋白的形式分泌到培养基中。 Western印迹显示这两个条带都是犬垂体蛋白提取物。

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《Experimental Animals》 |2010年第5期|共4页
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Osamu HASHIMOTO; Tomohiro YONEZAWA; Yuka SUGIYAMA; Mitsumori KAWAMINAMI; Yoshihisa HASEGAWA;
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中图分类动物学;
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Molecular Cloning and Expression of Canine Prolactin Gene

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