Molecular Cloning and Expression of Canine Prolactin Gene

Osamu HASHIMOTO; rnTomohiro YONEZAWA; rnYuka SUGIYAMA; Mitsumori KAWAMINAMI; rnYoshihisa HASEGAWA

首页> 外文期刊>Experimental Animals >Molecular Cloning and Expression of Canine Prolactin Gene

【24h】

Molecular Cloning and Expression of Canine Prolactin Gene

机译：犬催乳素基因的分子克隆与表达

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We isolated canine prolactin cDNA from a dog pituitary cDNA library. The 930-bp nucleotide sequence covered the entire open reading frame encoding the putative 229 amino acids. It was located in chromosome 35, and had five exons. The amino acid sequence was highly homologous to the feline and porcine sequences. To generate recombinant canine prolactin, plasmids for full-length canine prolactin were constructed and transfected into the mammalian HEK293 cell line. The recombinant prolactin was secreted into the medium as an N-linked glycosylated (31 kDa) or non-glycosylated (27 kDa) protein. Western blotting revealed both of these bands were canine pituitary protein extracts.

机译：我们从狗垂体cDNA文库中分离出犬催乳素cDNA。 930 bp的核苷酸序列覆盖了编码假定的229个氨基酸的整个开放阅读框。它位于第35号染色体上，有五个外显子。氨基酸序列与猫和猪序列高度同源。为了产生重组犬催乳素，构建了全长犬催乳素的质粒，并将其转染到哺乳动物HEK293细胞系中。重组催乳素以N-联糖基化（31 kDa）或非糖基化（27 kDa）蛋白的形式分泌到培养基中。蛋白质印迹显示这两个条带都是犬垂体蛋白提取物。

著录项

来源
《Experimental Animals》 |2010年第5期|p.643-646|共4页
作者
Osamu HASHIMOTO; rnTomohiro YONEZAWA; rnYuka SUGIYAMA; Mitsumori KAWAMINAMI; rnYoshihisa HASEGAWA;
展开▼
作者单位

Laboratory of Experimental Animal Science, Kitasato University, School of Veterinary Medicine, 35-1 Higashi 23, Towada, Aomori 034-8628, Japan;

rnLaboratories of Veterinary Physiology, Kitasato University,School of Veterinary Medicine, Towada, Aomori 034-8628, Japan;

rnLaboratories of Experimental Animal Science and School of Veterinary Medicine, Towada, Aomori 034-8628, Japan;

rnLaboratories of Veterinary Physiology, Kitasato University,School of Veterinary Medicine, Towada, Aomori 034-8628, Japan;

rnLaboratories of Experimental Animal Science and School of Veterinary Medicine, Towada, Aomori 034-8628, Japan;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
dog; pituitary; recombinant protein;

机译：狗;垂体;重组蛋白;

相似文献

外文文献
中文文献
专利

1. Molecular Cloning and Expression of Canine Prolactin Gene [J] . Osamu HASHIMOTO, Tomohiro YONEZAWA, Yuka SUGIYAMA, Experimental Animals . 2010,第5期

机译：犬催乳素基因的分子克隆与表达
2. Goldfish kisspeptin: molecular cloning, tissue distribution of transcript expression, and stimulatory effects on prolactin, growth hormone and luteinizing hormone secretion and gene expression via direct actions at the pituitary level. [J] . Yang B, Jiang Q, Chan General and comparative endocrinology . 2010,第1期

机译：金鱼Kisspeptin：通过垂体的直接作用，进行分子克隆，转录本表达的组织分布以及对催乳素，生长激素和促黄体激素分泌和基因表达的刺激作用。
3. Molecular cloning of the canine fragile histidine triad (FHIT) gene and fhit protein expression in canine peripheral blood mononuclear cells. [J] . Hiraoka H., Minami K., Kaneko N., The Journal of Veterinary Medical Science . 2009,第5期

机译：犬外周血单核细胞中犬脆性组氨酸三联体（ FHIT ）基因的分子克隆和fhit蛋白表达。
4. MOLECULAR CLONING AND HETEROLOGOUS EXPRESSION OF TWO CORIOLUS VERSICOLOR LACCASE ISOENZYME GENES IN PICHIA PASTORIS [C] . He Liyan, Xie Huifang, Cao Fuliang, International symposium on emerging technologies of pulping and papermaking;ISETPP . 2010

机译：斑节对虾两个变色斑马鱼胶酶同工酶基因的分子克隆和异源表达
5. Molecular cloning and sequencing of thioesterase B cDNA and hormonal induction of this thioesterase in the uropygial gland of mallard duck and molecular cloning of genes whose expression are triggered uniquely during appressorium formation in Colletotrichum gloeosporioides induced by its plant host signal. [D] . Hwang, Cheng-Shine. 1992

机译：野鸭鸭尿腺中硫酯酶B cDNA的分子克隆和测序以及该硫酯酶的激素诱导作用以及其植物宿主信号诱导的炭疽菌（Appletetrichum gloeosporioides）顶生过程中独特地触发其表达的基因的分子克隆。
6. Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor ofMacrophage [O] . Shintaro TOMURA, Mona UCHIDA, Tomohiro YONEZAWA, 2014

机译：犬凋亡抑制物的分子克隆与基因表达。巨噬细胞
7. Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage [O] . Shintaro TOMURA, Mona UCHIDA, Tomohiro YONEZAWA, 2014

机译：甘草菜凋亡抑制剂的分子克隆与基因表达
8. Molecular Approaches to Drug Abuse Research. Volume 1. Receptor Cloning,Neurotransmitter Expression, and Molecular Genetics [R] . Lee, T. N. H. 1991

机译：药物滥用研究的分子方法。第1卷。受体克隆，神经递质表达和分子遗传学

Molecular Cloning and Expression of Canine Prolactin Gene

摘要

著录项

相似文献

相关主题

期刊订阅