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首页> 外文期刊>Experimental diabetes research >Early Degenerative Effects of Diabetes Mellitus on Pancreas, Liver, and Kidney in Rats: An Immunohistochemical Study
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Early Degenerative Effects of Diabetes Mellitus on Pancreas, Liver, and Kidney in Rats: An Immunohistochemical Study

机译：糖尿病对大鼠胰腺，肝脏和肾脏的早期变性作用：一项免疫组织化学研究

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Liver and kidney commonly affected by diabetes in chronic cases but pathogenetic mechanisms are not fully understood in early stages of the disease. The aim of this study was to investigate the immunohistochemical expression of caspase-3, cyclooxygenase (COX)-1 and-2, calcium sensing receptor (CSR), and hypoxia inducible factor-1α(HIF-1α) in pancreas, liver, and kidney in streptozotocin (STZ) induced DM. Study group (n= 6) were received streptozotocin (50 mg/kg) and control group (n= 6) physiologic saline. The blood glucose and ketonuria were measured, and necropsy was performed on them on third, fourth, and fifth days. Immunohistochemistry revealed that marked increase in caspase-3 reaction pancreas, liver, and kidney in the study group than control group. COX-1 slightly increased in these organs in study group compared to controls. Immunohistochemically COX-2 reaction was markedly positive in liver and kidney, but slightly increased in pancreas. The most increased reaction was observed in CRS and all organs were markedly positive. HIF-1αexpression was also increased but the reaction was more severe in pancreas than liver and kidney. This study indicated that degeneration starts in organs in early stages of the disease and the most effective route for degeneration related to increase of calcium influx and hypoxia upon cells in DM.
机译：在慢性病例中，肝和肾通常受糖尿病影响，但在疾病的早期阶段尚不完全了解其发病机理。这项研究的目的是研究胰腺，肝脏和肝脏中caspase-3，环氧合酶（COX）-1和-2，钙敏感受体（CSR）和缺氧诱导因子1α（HIF-1α）的免疫组织化学表达。肾脏在链脲佐菌素（STZ）诱导的DM中。研究组（n = 6）接受链脲佐菌素（50μg/ kg），对照组（n = 6）接受生理盐水。测量血糖和酮尿症，并在第三，第四和第五天对其进行尸检。免疫组织化学显示，研究组的caspase-3反应胰腺，肝脏和肾脏明显高于对照组。与对照组相比，研究组这些器官中的COX-1略有增加。免疫组织化学COX-2反应在肝和肾中显着阳性，但在胰腺中略有增加。在CRS中观察到反应增加最多，所有器官均为阳性。 HIF-1α的表达也增加，但胰腺中的反应比肝和肾更严重。这项研究表明，变性是在疾病的早期阶段开始，并且最有效的变性途径与DM细胞中钙流入和缺氧的增加有关。

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