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Ribozyme-catalyzed trimming reactions andthe direct role of Mg2+ ions in the cleavage of RNA

机译：核酶催化的修整反应和Mg2 +离子在RNA切割中的直接作用

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In the hypothetical RNA world divalent Mg2+ ions were exploited for cleavage (or ligation) ofribonucleic acids. Although some Mg2+ ions are involved in forming the tertiary structures ofRNAs, the key Mg2+ ions are directly involved in catalysis not only in the case of hammerhead ribozymes but also in the case of Tetrahymena and, possibly, other types of ribozyme. RNAcomponents bind the indispensable Mg2+ ions to the phosphodiester bonds that are being broken (or formed). Our analysis indicates that the chemical cleavage step of reactions catalyzed by the hammerhead ribozyme does not appear to have been perfected and, thus, it seems possible to create RNA-cleaving agents that are significantly more active than the standard hammerhead ribozyme. Moreover, RNA-cleaving mechanisms might converge as one unique and universal mechanism, exploited not only by various kinds of ribozyme but also by artificially created metal-ion-dependent DNAzymes and other RNA-cleaving agents that are yet to be identified.
机译：在假设的RNA世界中，利用二价Mg2 +离子切割（或连接）核糖核酸。尽管某些Mg2 +离子参与了RNA的三级结构的形成，但关键的Mg2 +离子不仅直接参与了锤头状核酶，而且还参与了四膜虫以及其他类型的核酶的催化。 RNA成分将必不可少的Mg2 +离子与被破坏（或形成）的磷酸二酯键结合。我们的分析表明，锤头状核酶催化的化学裂解步骤似乎尚未完善，因此，似乎有可能创造出比标准锤头状核酶活性更高的RNA裂解剂。此外，RNA裂解机制可能会融合为一种独特而通用的机制，不仅被各种核酶所利用，而且还被人工创建的依赖金属离子的DNA酶和其他尚未被鉴定的RNA裂解剂所利用。

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