Efficient in vivo expression of a reporter gene in ratbrain after injection of recombinant replication-deficient Semliki Forest virus

Dr.; Kenneth Lundstrom

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Efficient in vivo expression of a reporter gene in ratbrain after injection of recombinant replication-deficient Semliki Forest virus

机译：注射重组复制缺陷型Semliki Forest病毒后，鼠脑中报告基因的高效体内表达

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Recombinant replication-deficient Semliki Forest virus (SFV) expressing bacterial ? -galactosidase was injected into the amygdala and striatum of male Wistar rats. Reporter gene expression was detected up to 28 days post-injection. The maximal expression levels were obtained 1-2 days post- injection. In situ hybridization studies demonstrated high expression of LacZ mRNA until day 2, but no signal was detected 4 days post-injection. No significant change in body weight and temperature, exploratory locomotor behavior and forced motor performances were observed after SFV-LacZ injections. The neuronal gene transfer with SFV vectors did not trigger any major cell toxicity.

机译：表达细菌的重组复制缺陷型Semliki森林病毒（SFV）？将半乳糖苷酶注射入雄性Wistar大鼠的杏仁核和纹状体中。注射后长达28天检测到报告基因的表达。注射后1-2天获得最大表达水平。原位杂交研究表明，LacZ mRNA高表达直至第2天，但注射后4天未检测到信号。注射SFV-LacZ后，未观察到体重和体温，探索性运动行为和强迫运动表现的显着变化。 SFV载体的神经元基因转移未触发任何主要的细胞毒性。

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《Gene Therapy and Molecular Biology》 |1999年第3期|共9页
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Dr.; Kenneth Lundstrom;
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Efficient in vivo expression of a reporter gene in ratbrain after injection of recombinant replication-deficient Semliki Forest virus

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