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Intrathecal injection: An effective technique to implement gene transfection inside central nervous system

机译：鞘内注射：在中枢神经系统内部进行基因转染的有效技术

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Spinal cord injury (SCI) will lead to functional deficit of limbs and sometimes even affect the patient’s life. Now, little measures are effective except administration of great dosage of methylprednisolone and decompression surgery in early stage. But general application of the drug may cause side effects and operation can only remove the mass that compress the dura or spinal cord. More safe and effective methods will be needed during the operation. We use intrathecal injection to implement gene transfection inside central nervous system (CNS). Through gene delivery technique, continuous expression of functional protein, such as neuro-protective factor at local site may be possible. In mRNA level, expression of reporter gene was demonstrated at the 2-week point by display of Lac Z-mRNA via RT-PCR. However, no bands of control group can be detected by this way except β-actin. Experiment group represents blue dyed cell (neuron or glial cell) in spinal cord, but control group doesn’t. The tissue proximal to injection point represents more positive cells than that of distal to the point (according to quantity analysis). The intrathecal injection technique applied in the current study make regional gene transfection within CNS system possible.
机译：脊髓损伤（SCI）将导致四肢功能障碍，有时甚至会影响患者的生活。现在，除了大剂量的甲泼尼龙和早期减压手术外，几乎没有什么有效的措施。但是药物的一般应用可能会引起副作用，并且操作只能去除压缩硬脑膜或脊髓的肿块。手术期间将需要更安全有效的方法。我们使用鞘内注射在中枢神经系统（CNS）内部实施基因转染。通过基因传递技术，功能蛋白（例如神经保护因子）在局部部位的连续表达是可能的。在mRNA水平上，通过RT-PCR显示Lac Z-mRNA在2周点证实了报告基因的表达。但是，除β-肌动蛋白外，这种方法无法检测到对照组的条带。实验组代表脊髓中的蓝色染色细胞（神经元或神经胶质细胞），而对照组则没有。注射点附近的组织代表的阳性细胞多于注射点远端的组织（根据数量分析）。当前研究中使用的鞘内注射技术使CNS系统内区域基因转染成为可能。

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