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Study of genetic diversity of Iranian indigenous buffalo populations using microsatellite markers

机译：利用微卫星标记研究伊朗土著水牛种群的遗传多样性

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Indigenous buffalo breeds represent a unique genetic resource, and understanding their variability, population structure and breeding units is important for their sustainable conservation. In the present study the genetic structure of Iranian buffalo populations was analyzed using ten microsatellite markers. Two hundred hair samples were collected and DNA was extracted using modified salting out method. After Polymerase Chain Reaction (PCR), the PCR products were electrophoresed using 9% polyacrylamide gel. Fifty- nine alleles were observed for all the loci. The average number of alleles was 5.90 and the effective average number of alleles was 4.86. The high level of mean heterozygosity index between three populations indicate that the genetic diversity is high in within and between populations. The mean of polymorphism information content (PIC) value for all loci was 0.70. The FST value for the total loci was 0.01, indicating a very low level of genetic structure among populations. The genetic structure AMOVA analysis showed that about 3% of the total genetic variation was explained by population differences and 97 percent was corresponded to differences among individuals. The obtained results at the present study indicated that characterization of genetic diversity by employing molecular tools is a prerequisite in developing strategies for conservation and utilization of buffalo genetic resources.
机译：土著水牛品种代表着独特的遗传资源，了解它们的变异性，种群结构和繁殖单位对其可持续保护很重要。在本研究中，使用十个微卫星标记分析了伊朗水牛种群的遗传结构。收集了200个头发样本，并使用改良的盐析方法提取了DNA。聚合酶链反应（PCR）后，PCR产物使用9％聚丙烯酰胺凝胶电泳。所有基因座均观察到59个等位基因。等位基因的平均数为5.90，有效等位基因的平均数为4.86。三个种群之间的平均杂合度指数较高，表明种群内部和种群之间的遗传多样性较高。所有基因座的多态信息含量（PIC）值的平均值为0.70。总基因座的FST值为0.01，表明人群中的遗传结构水平非常低。遗传结构AMOVA分析表明，总遗传变异中约3％由种群差异解释，而97％与个体差异相对应。在本研究中获得的结果表明，利用分子工具表征遗传多样性是制定水牛遗传资源保护和利用策略的先决条件。

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