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首页> 外文期刊>Genes and Nutrition >Resveratrol protects ROS-induced cell death by activating AMPK in H9c2 cardiac muscle cells
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Resveratrol protects ROS-induced cell death by activating AMPK in H9c2 cardiac muscle cells

机译：白藜芦醇通过激活H9c2心肌细胞中的AMPK保护ROS诱导的细胞死亡

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摘要

Resveratrol, one of polyphenols derived from red wine, has been shown to protect against cell death, possibly through the association with several signaling pathways. Currently numerous studies indicate that cardiovascular diseases are linked to the release of intracellular reactive oxygen species (ROS) often generated in states such as ischemia/reperfusion injury. In the present study, we investigated whether resveratrol has the capability to control intracellular survival signaling cascades involving AMP-activated kinase (AMPK) in the inhibitory process of cardiac injury. We hypothesized that resveratrol may exert a protective effect on damage to heart muscle through modulating of the AMPK signaling pathway. We mimicked ischemic conditions by inducing cell death with H2O2 in H9c2 muscle cells. In this experiment, resveratrol induced strong activation of AMPK and inhibited the occurrence of cell death caused by treatment with H2O2. Under the same conditions, inhibition of AMPK using dominant negative AMPK constructs dramatically abolished the effect of resveratrol on cell survival in H2O2-treated cardiac muscle cells. These results indicate that resveratrol-induced cell survival is mediated by AMPK in H9c2 cells and may exert a novel therapeutic effect on oxidative stress induced in cardiac disorders.
机译：白藜芦醇，一种从红酒中提取的多酚，已被证明可以防止细胞死亡，可能是通过与几种信号通路的结合来实现的。当前，大量研究表明，心血管疾病与通常在缺血/再灌注损伤等状态下产生的细胞内活性氧（ROS）释放有关。在本研究中，我们调查了白藜芦醇是否具有控制心脏损伤抑制过程中涉及AMP活化激酶（AMPK）的细胞内生存信号转导级联的能力。我们假设白藜芦醇可以通过调节AMPK信号通路对心肌造成保护作用。我们通过在H9c2肌肉细胞中用H2O2诱导细胞死亡来模拟缺血性疾病。在该实验中，白藜芦醇诱导了AMPK的强烈活化，并抑制了H2O2处理引起的细胞死亡。在相同条件下，使用显性阴性AMPK构建体对AMPK的抑制作用显着消除了白藜芦醇对H2O2处理的心肌细胞中细胞存活的影响。这些结果表明白藜芦醇诱导的细胞存活是由H9c2细胞中的AMPK介导的，并且可能对心脏疾病中诱导的氧化应激产生新的治疗作用。

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