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首页> 外文期刊>Genes and Nutrition >Validating nutrient-related gene expression changes from microarrays using RT2 PCR-arrays
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Validating nutrient-related gene expression changes from microarrays using RT2 PCR-arrays

机译：使用RT2 PCR芯片验证微阵列中营养相关基因的表达变化

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摘要

Microarray technology allows us to perform high-throughput screening of changes in gene expression. The outcome of microarray experiments largely depends on the applied analysis methods and cut-off values chosen. Results are often required to be verified using a more sensitive detection technique, such as quantitative real-time PCR (qPCR or RT-PCR). Throughout the years, this technique has become a de facto golden standard. Individual qPCRs are time-consuming, but the technology to perform high-throughput qPCR reactions has become available through PCR-arrays that allow up to 384 PCR reactions simultaneously. Our current aim was to investigate the usability of a RT2 Profiler? PCR-array as validation in a nutritional intervention study, where the measured changes in gene expression were low. For some differentially expressed genes, the PCR-array confirmed the microarray prediction, though not for all. Furthermore, the PCR-array allowed picking up the expression of genes that were not measurable on the microarray platform but also vice versa. We conclude that both techniques have their own (dis)advantages and specificities, and for less pronounced changes using both technologies may be useful as complementation rather than validation.
机译：微阵列技术使我们能够对基因表达的变化进行高通量筛选。微阵列实验的结果很大程度上取决于应用的分析方法和选择的临界值。通常需要使用更灵敏的检测技术来验证结果，例如定量实时PCR（qPCR或RT-PCR）。多年来，这项技术已成为事实上的黄金标准。单个qPCR很耗时，但是通过可同时进行多达384个PCR反应的PCR阵列，可以进行高通量qPCR反应的技术。我们当前的目标是调查RT2 Profiler的可用性？ PCR阵列作为营养干预研究的验证，其中基因表达的测量变化很小。对于某些差异表达的基因，PCR阵列证实了微阵列的预测，尽管不是全部。此外，PCR阵列允许拾取在微阵列平台上无法测量的基因表达，反之亦然。我们得出的结论是，这两种技术都有其自身的（缺点）优势和特异性，对于不太明显的变更，使用这两种技术可能会作为补充而不是验证而有用。

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