...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genes and Nutrition >Angiogenesis in Balb/c mice under beta-carotene supplementation in diet
【24h】

Angiogenesis in Balb/c mice under beta-carotene supplementation in diet

机译：饮食中β-胡萝卜素补充对Balb / c小鼠血管生成的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Angiogenesis is a process of new blood vessel formation from pre-existing ones. The most important steps in angiogenesis include detachment, proliferation, migration, homing and differentiation of vascular wall cells, which are mainly endothelial cells and their progenitors. The study focused on the effect of beta-carotene (BC) supplementation (12,000?mg/kg) in the diet on angiogenesis in Balb/c mice. Female Balb/c mice were fed for 5?weeks with two different diets: with BC or without BC supplementation. After 4?weeks of feeding, Balb/c mice were injected subcutaneously with two matrigel plugs with or without basic fibroblast growth factor (bFGF). Six days later, the animals were killed, and the matrigel plugs were used for immunohistochemical staining with CD31 antibody and for gene expression analysis. Microarray and Real-Time PCR data showed down-regulation of genes involved in proliferation and up-regulation of genes encoding inhibitors of apoptosis, proteins regulating cell adhesion, matrix-degrading enzymes and proteins involved in the VEGF pathway. The results of this study demonstrated that BC proangiogenic activity (with or without bFGF) in vivo seemed to be more significantly associated with cells’ protection from apoptosis and their stimulation of chemotaxis/homing than cell proliferation.
机译：血管生成是从已有血管生成新血管的过程。血管生成中最重要的步骤包括血管壁细胞的分离，增殖，迁移，归巢和分化，血管壁细胞主要是内皮细胞及其祖细胞。该研究集中于饮食中补充β-胡萝卜素（BC）（12,000？mg / kg）对Balb / c小鼠血管生成的影响。用两种不同的饮食喂养雌性Balb / c小鼠5周：添加BC或不添加BC。喂养4周后，给Balb / c小鼠皮下注射两个带有或不带有碱性成纤维细胞生长因子（bFGF）的基质胶塞。六天后，将动物处死，将基质胶塞用于CD31抗体的免疫组织化学染色和基因表达分析。微阵列和实时PCR数据显示，与增殖有关的基因下调，而编码细胞凋亡抑制剂，调节细胞粘附的蛋白，基质降解酶和与VEGF途径有关的蛋白的基因上调。这项研究的结果表明，体内的BC促血管生成活性（有或没有bFGF）似乎比细胞增殖与保护细胞免于凋亡以及刺激趋化性/归巢的能力显着相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号