...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genome Biology and Evolution >Genome Sequencing of Giardia lamblia Genotypes A2 and B Isolates (DH and GS) and Comparative Analysis with the Genomes of Genotypes A1 and E (WB and Pig)
【24h】

Genome Sequencing of Giardia lamblia Genotypes A2 and B Isolates (DH and GS) and Comparative Analysis with the Genomes of Genotypes A1 and E (WB and Pig)

机译：贾第鞭毛虫基因型A2和B分离株（DH和GS）的基因组测序以及与基因型A1和E（WB和猪）的基因组比较分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Giardia lamblia (syn G. intestinalis, G. duodenalis) is the most common pathogenic intestinal parasite of humans worldwide and is a frequent cause of endemic and epidemic diarrhea. G. lamblia is divided into eight genotypes (A–H) which infect a wide range of mammals and humans, but human infections are caused by Genotypes A and B. To unambiguously determine the relationship among genotypes, we sequenced GS and DH (Genotypes B and A2) to high depth coverage and compared the assemblies with the nearly completed WB genome and draft sequencing surveys of Genotypes E (P15; pig isolate) and B (GS; human isolate). Our results identified DH as the smallest Giardia genome sequenced to date, while GS is the largest. Our open reading frame analyses and phylogenetic analyses showed that GS was more distant from the other three genomes than any of the other three were from each other. Whole-genome comparisons of DH_A2 and GS_B with the optically mapped WB_A1 demonstrated substantial synteny across all five chromosomes but also included a number of rearrangements, inversions, and chromosomal translocations that were more common toward the chromosome ends. However, the WB_A1/GS_B alignment demonstrated only about 70% sequence identity across the syntenic regions. Our findings add to information presented in previous reports suggesting that GS is a different species of Giardia as supported by the degree of genomic diversity, coding capacity, heterozygosity, phylogenetic distance, and known biological differences from WB_A1 and other G. lamblia genotypes.
机译：贾第鞭毛虫（S. G. intestinalis，G. duodenalis）是全世界人类最常见的致病性肠道寄生虫，是地方性和流行性腹泻的常见原因。 G. lamblia分为八种基因型（A–H），可感染多种哺乳动物和人类，但人类感染是由基因型A和B引起的。为了明确确定基因型之间的关系，我们对GS和DH进行了测序（基因型B和A2）进行深度覆盖，并将装配体与几乎完成的WB基因组和基因型E（P15;猪分离株）和B（GS;人分离株）的测序调查草案进行比较。我们的结果确定了DH是迄今为止测序的最小的贾第鞭毛虫基因组，而GS是最大的。我们的开放阅读框分析和系统发育分析表明，GS与其他三个基因组的距离比其他三个基因组的距离更远。 DH_A2和GS_B与光学映射的WB_A1的全基因组比较表明，在所有五个染色体上都有相当的同义性，但是还包括许多重排，倒位和染色体易位，这些染色体向染色体末端更常见。但是，WB_A1 / GS_B比对显示整个同义区域仅约70％的序列同一性。我们的发现增加了先前报告中提供的信息，表明基因组多样性程度，编码能力，杂合性，系统发生距离以及与WB_A1和其他兰博拉氏菌基因型的已知生物学差异支持了GS是贾第鞭毛虫的不同物种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号