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首页> 外文期刊>Genetics and Molecular Research >Effect of total peony glucoside pretreatment on NF-κB and ICAM-1 expression in myocardial tissue of rat with myocardial ischemia-reperfusion injury
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Effect of total peony glucoside pretreatment on NF-κB and ICAM-1 expression in myocardial tissue of rat with myocardial ischemia-reperfusion injury

机译：药总苷对心肌缺血再灌注损伤大鼠心肌组织NF-κB和ICAM-1表达的影响

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Early recovery of myocardial perfusion is beneficial for myocardial ischemia. However, ischemia-reperfusion (I/R) may exacerbate myocardial injury. Research shows that total peony glucoside (TPG) can inhibit ischemic myocardial cell apoptosis. However, whether it can ameliorate I/R injury remains poorly understood. This study explored the effect of TPG pretreatment on I/R, through nuclear factor-kappa B (NF-κB) and intercellular adhesion molecule-1 (ICAM-1) expressions in I/R-affected myocardium. Healthy 7-week-old male Sprague Dawley rats were randomly categorized into sham operation (A), modeling (B), and 100, 200, and 400 mg/kg TPG pretreatment groups (C, D, and E, respectively), with 20 rats in each group. I/R rat models were designed by ligating left anterior descending coronary artery for 30 min to induce ischemia and for 120 min to induce reperfusion. Serum interleukin 6 (IL-6) and interleukin 8 (IL-8) levels were measured using enzyme linked immunosorbent assay. NF-κB and ICAM-1 mRNA and protein expressions were detected through RT-PCR and western blot analysis, respectively. Compared to group A, serum IL-6 and IL-8 levels of group B elevated significantly (P 0.05), whereas NF-κB and ICAM-1 mRNA and protein expressions increased in the myocardium (P 0.05). Serum IL-6 and IL-8 levels, and NF-κB and ICAM-1 mRNA and protein expressions, in myocardium of TPG groups reduced in a dose-dependent manner. Therefore, TPG pretreatment could alleviate myocardium reperfusion injury in I/R rat models by reducing NF-κB and ICAM-1 mRNA and protein expressions and cytokine secretions. This mechanism could be associated with the inhibition of NF-κB activation and downregulation of ICAM-1 expression.
机译：心肌灌注的早期恢复有益于心肌缺血。但是，缺血再灌注（I / R）可能会加剧心肌损伤。研究表明，总牡丹苷（TPG）可以抑制缺血性心肌细胞凋亡。但是，是否可以减轻I / R伤害仍然知之甚少。这项研究探讨了TPG预处理通过受I / R影响的心肌中核因子-κB（NF-κB）和细胞间粘附分子-1（ICAM-1）的表达对I / R的影响。将健康的7周大雄性Sprague Dawley大鼠随机分为假手术（A），模型（B）和100、200和400 mg / kg TPG预处理组（分别为C，D和E），分别为每组20只大鼠。通过结扎左冠状动脉前降支30分钟以诱导缺血和120分钟以诱导再灌注来设计I / R大鼠模型。使用酶联免疫吸附测定法测量血清白介素6（IL-6）和白介素8（IL-8）的水平。通过RT-PCR和western blot分析分别检测NF-κB和ICAM-1的mRNA和蛋白表达。与A组相比，B组血清IL-6和IL-8水平显着升高（P <0.05），而心肌中NF-κB和ICAM-1mRNA和蛋白表达升高（P <0.05）。 TPG组心肌中的血清IL-6和IL-8水平以及NF-κB和ICAM-1 mRNA和蛋白表达呈剂量依赖性降低。因此，TPG预处理可以通过减少NF-κB和ICAM-1 mRNA和蛋白表达以及细胞因子的分泌来减轻I / R大鼠模型的心肌再灌注损伤。该机制可能与抑制NF-κB活化和下调ICAM-1表达有关。

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