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The global expression profiling in esophageal squamous cell carcinoma

机译：食管鳞状细胞癌的整体表达谱

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Esophageal squamous cell carcinoma (ESCC) is the dominant subtype of esophageal cancer worldwide. This study aimed to explore the aberrant global expression profiling and construct regulatory network in ESCC for understanding tumorigenesis of ESCC. The expression pattern of long non-coding RNA (lncRNA), microRNA (miRNA) and mRNA was measured by RNA-sequencing in ESCC. Differentially expressed lncRNAs/miRNAs/mRNAs (DELs/DEMs/DEMIs) were identified in ESCC. DEMIs-DEMs network was constructed; hsa-miR-424-5p and hsa-miR-450b-5p were the hub miRNAs in the network, which negatively regulated 19 and 17 DEMs. DEMs targeted by DEMIs were significantly enriched in MAPK signaling pathway, pathways in cancer and focal adhesion signaling pathway. The expression of candidate DEMs and DEMIs in ESCC were validated through quantitative real-time polymerase chain reaction and microarray expression profiling analyses, and the results were generally consistent with our bioinformatics analysis. Our results might provide useful information for exploring the tumorigenesis mechanism and potentially therapeutic targets in ESCC.
机译：食管鳞状细胞癌（ESCC）是全世界食管癌的主要亚型。这项研究旨在探索异常的全球表达谱，并在ESCC中构建调控网络，以了解ESCC的肿瘤发生。在ESCC中通过RNA测序测量长非编码RNA（lncRNA），微小RNA（miRNA）和mRNA的表达模式。在ESCC中鉴定了差异表达的lncRNA / miRNA / mRNA（DEL / DEM / DEMI）。 DEMIs-DEMs网络已建立; hsa-miR-424-5p和hsa-miR-450b-5p是网络中的集线器miRNA，对19和17个DEM产生负调控。 DEMI靶向的DEM在MAPK信号通路，癌症通路和粘着斑信号通路中显着丰富。通过定量实时聚合酶链反应和微阵列表达谱分析验证了候选DEM和DEMI在ESCC中的表达，其结果与我们的生物信息学分析基本一致。我们的结果可能为探索ESCC的肿瘤发生机制和潜在治疗靶点提供有用的信息。

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