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首页> 外文期刊>G3: Genes, Genomes, Genetics >A Resource for the Allele-Specific Analysis of DNA Methylation at Multiple Genomically Imprinted Loci in Mice
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A Resource for the Allele-Specific Analysis of DNA Methylation at Multiple Genomically Imprinted Loci in Mice

机译：在小鼠中多个基因组印迹基因座的DNA甲基化等位基因特异性分析的资源。

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Genomically imprinted loci are expressed mono-allelically, dependent upon the parent of origin. Their regulation not only illuminates how chromatin regulates gene expression but also how chromatin can be reprogrammed every generation. Because of their distinct parent-of-origin regulation, analysis of imprinted loci can be difficult. Single nucleotide polymorphisms (SNPs) are required to accurately assess these elements allele specifically. However, publicly available SNP databases lack robust verification, making analysis of imprinting difficult. In addition, the allele-specific imprinting assays that have been developed employ different mouse strains, making it difficult to systemically analyze these loci. Here, we have generated a resource that will allow the allele-specific analysis of many significant imprinted loci in a single hybrid strain of Mus musculus . This resource includes verification of SNPs present within 10 of the most widely used imprinting control regions and allele-specific DNA methylation assays for each gene in a C57BL/6J and CAST/EiJ hybrid strain background.
机译：基因印记的基因座根据来源母体单等位基因表达。它们的调控不仅阐明了染色质如何调控基因表达，而且阐明了如何一代代地重新编程染色质。由于其独特的原产地法规，因此很难分析印迹基因座。需要单核苷酸多态性（SNP）才能准确准确地评估这些元素等位基因。但是，公开可用的SNP数据库缺乏可靠的验证，因此难以分析印迹。此外，已开发的等位基因特异性印迹分析采用了不同的小鼠品系，因此难以系统地分析这些基因座。在这里，我们已经生成了一种资源，该资源将允许在小家鼠的一个杂交株中对许多重要的印迹基因座进行等位基因特异性分析。此资源包括对C57BL / 6J和CAST / EiJ杂种菌株背景中每个基因的10个最广泛使用的印迹控制区中存在的SNP进行验证，以及每个基因的等位基因特异性DNA甲基化测定。

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