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Complete mitochondrial genome sequence and gene organization of Chinese indigenous chickens with phylogenetic considerations

机译:考虑系统发育的中国本土鸡线粒体基因组序列和基因的完整组织

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In this study, we sequenced the complete mitochondrial DNA of Chinese indigenous Jinhu Black-bone and Rugao chickens. The two chicken mitochondrial genomes were deposited in GenBank under accession Nos. KP742951 and KR347464, respectively. The complete mitochondrial genomes of Jinhu Black-bone and Rugao chickens were sequenced and found to span 16,785 and 16,786 bp, respectively, and consisted of 22 tRNA genes, two rRNA genes (12S rRNA and 16S rRNA), 13 protein-coding genes, and one control region (D-loop). The majority of genes were positioned on the H-strand, and the ND6 and eight tRNA genes were found to be encoded on the L-strand. The mitogenomes showed a similar gene order to that of the published Gallus gallus genome, as neither included a control region. The overall base composition of the genome of the two chickens was A = 30.22/30.28%, G = 13.57/13.49%, T = 23.74/23.76%, and C = 32.48/32.48%. Nucleotide skewness of the coding strands of the two chicken genomes (AT-skew = 0.12, GC-skew = -0.41) was biased towards T and G. Phylogenetic analysis revealed 29 subspecies, and the molecular genetic relationship among the 29 subspecies was identical to that of traditional taxonomy.
机译:在这项研究中,我们对中国本土的金虎乌骨鸡和如uga鸡的线粒体DNA进行了完整测序。这两个鸡线粒体基因组分别存放在GenBank中,登录号为KP742951和KR347464。对金湖黑骨和如uga鸡的线粒体完整基因组进行测序,分别跨越16785bp和16786bp,包括22个tRNA基因,2个rRNA基因(12S rRNA和16S rRNA),13个蛋白质编码基因,一个控制区域(D环)。大多数基因位于H链上,并且发现ND6和8个tRNA基因在L链上编码。有丝分裂基因组显示出与已发表的鸡盖洛斯基因组相似的基因顺序,因为两者均未包含对照区。两只鸡的基因组的总体碱基组成为A = 30.22 / 30.28%,G = 13.57 / 13.49%,T = 23.74 / 23.76%,C = 32.48 / 32.48%。两个鸡基因组的编码链的核苷酸偏度(AT偏度= 0.12,GC偏度= -0.41)偏向T和G。系统进化分析显示29个亚种,并且这29个亚种之间的分子遗传关系与传统分类法。

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