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首页> 外文期刊>Genetics and Molecular Research >Identification of CD14 transcript in blood polymorphonuclear neutrophil leukocytes and functional variation in Holsteins
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Identification of CD14 transcript in blood polymorphonuclear neutrophil leukocytes and functional variation in Holsteins

机译：血液中性粒细胞中性粒细胞白细胞CD14转录本的鉴定及其功能变化

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Polymorphonuclear neutrophil (PMN) leukocytes are primary phagocytic cells of the bovine mammary gland and a first line of defense against invading pathogens during bovine mastitis infection. Cluster of differentiation 14 (CD14) is mainly expressed in macrophages and neutrophils and acts as a co-receptor that binds bacterial lipopolysaccharide (LPS) and recruits PMNs to CD14-LPS complexes in mammary epithelial cells. In this study, we identified a novel splice variant in PMNs, named CD14-SV, characterized by a deleted region from c.143-579 nt compared to the CD14 reference mRNA sequence. Moreover, a single nucleotide polymorphism (c.523 AG) in exon 2 of CD14 was identified and found to modify the secondary structure and hydrophilicity of the CD14 protein. Association analysis also showed that the milk somatic cell score, an indicator of mastitis, of cows with the GG genotype was lower than that of cows with the AA and AG genotypes. Our findings suggest that the expression of CD14 in bovine blood PMNs is regulated by alternative splicing, and that CD14-SV is a candidate functional marker that may influence mastitis-resistance in dairy cows.
机译：多形核中性白细胞（PMN）白细胞是牛乳腺的原代吞噬细胞，是抵抗牛乳腺炎感染期间病原体的第一道防线。分化簇14（CD14）主要在巨噬细胞和嗜中性粒细胞中表达，并作为结合受体，与细菌脂多糖（LPS）结合并将PMN募集到乳腺上皮细胞中的CD14-LPS复合物中。在这项研究中，我们在PMN中鉴定了一个新的剪接变体，命名为CD14-SV，其特征是与CD14参考mRNA序列相比，来自c.143-579 nt的缺失区域。此外，鉴定出CD14外显子2中的单核苷酸多态性（c.523 A> G），发现其修饰CD14蛋白的二级结构和亲水性。关联分析还显示，GG基因型奶牛的乳体细胞得分（是乳腺炎的指标）低于AA和AG基因型奶牛。我们的发现表明，牛血液PMNs中CD14的表达受其他剪接调控，而CD14-SV是可能影响奶牛乳腺炎抵抗力的候选功能标记。

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