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首页> 外文期刊>Genetics and Molecular Research >Extracellular enzymatic profiles and taxonomic identification of endophytic fungi isolated from four plant species
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Extracellular enzymatic profiles and taxonomic identification of endophytic fungi isolated from four plant species

机译：四种植物内生真菌的细胞外酶学特征和分类学鉴定

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Plants of medicinal and economic importance have been studied to investigate the presence of enzyme-producing endophytic fungi. The characterization of isolates with distinct enzyme production potential may identify suitable alternatives for specialized industry. At Universidade Estadual de Maringá Laboratory of Microbial Biotechnology, approximately 500 isolates of endophytic fungi have been studied over the last decade from various host plants, including medicinally and economically important species, such as Luehea divaricata (Martius et Zuccarini), Trichilia elegans A. Juss, Sapindus saponaria L., Piper hispidum Swartz, and Saccharum spp. However, only a fraction of these endophytes have been identified and evaluated for their biotechnological application, having been initially grouped by morphological characteristics, with at least one representative of each morphogroup tested. In the current study, several fungal strains from four plants (L. divaricata, T. elegans, S. saponaria, and Saccharum spp) were identified by ribosomal DNA typing and evaluated semi-quantitatively for their enzymatic properties, including amylase, cellulase, pectinase, and protease activity. Phylogenetic analysis revealed the presence of four genera of endophytic fungi (Diaporthe, Saccharicola, Bipolaris, and Phoma) in the plants examined. According to enzymatic tests, 62% of the isolates exhibited amylase, approximately 93% cellulase, 50% pectinase, and 64% protease activity. Our results verified that the composition and abundance of endophytic fungi differed between the plants tested, and that these endophytes are a potential enzyme production resource of commercial and biotechnological value.
机译：已经研究了具有药用和经济重要性的植物，以研究产生酶的内生真菌的存在。具有独特的酶生产潜力的分离株的表征可以为专业化工业确定合适的替代品。在过去的十年中，马林大学微生物生物技术实验室研究了大约500种内生真菌的分离株，这些分离自多种寄主植物，包括药用和经济上重要的物种，如Luehea divaricata（Martius et Zuccarini），Trichilia elegans A. Juss ，Sapindus saponaria L.，Piper hispidum Swartz和Saccharum spp。但是，这些内生真菌中只有一小部分已经被鉴定并评估了它们的生物技术应用，并最初按形态特征进行了分组，每种被测形态组至少有一个代表。在当前的研究中，通过核糖体DNA分型鉴定了来自四种植物（L. divaricata，T。elegans，S。saponaria和Saccharum spp）的几种真菌菌株，并对它们的酶学性质进行了半定量评估，包括淀粉酶，纤维素酶，果胶酶。和蛋白酶活性。系统发育分析表明，在所检查的植物中存在四个内生真菌（Diaporthe，Saccharicola，Bipolaris和Phoma）。根据酶促测试，分离物的62％显示出淀粉酶，约93％的纤维素酶，50％的果胶酶和64％的蛋白酶活性。我们的结果证实了所测试的植物之间内生真菌的组成和丰度有所不同，并且这些内生真菌是具有商业和生物技术价值的潜在酶生产资源。

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