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Analysis of floral transcription factors from Lycoris longituba

机译：长叶石蒜的花转录因子分析

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Transcription factors (TFs) are proteins that bind to specific promoter regions of their target genes and regulate gene transcription. Many of these factors have been found to influence flowering. Lycoris longituba exhibits a great deal of diversity in flower color and flower form, making it a suitable model for the study of floral development. We have identified 338 putative TFs from more than thirty thousand ESTs sequenced from the floral tissue of L. longituba, and validated them using real-time RT-PCR. Fifty-one of the TFs were recognized as being potentially flower-specific, and the expression patterns of some of them during six flowering phases have been elucidated. Homolog annotation and phylogenetic analysis revealed that some TFs that belong to several TF families, such as MADS, MYB-related, NAC, and ABI3-VP1, were suggested to play important roles in the flowering process. Our dataset may be used to identify priority target TF genes for further study.
机译：转录因子（TFs）是与目标基因的特定启动子区域结合并调节基因转录的蛋白质。已经发现许多这些因素影响开花。长寿石蒜在花的颜色和花形上表现出很大的多样性，使其成为研究花发育的合适模型。我们从长叶杜鹃的花组织中测序的三万多个EST中鉴定出338个推定的TF，并使用实时RT-PCR对其进行了验证。 51个TF被认为具有潜在的花朵特异性，并且已经阐明了其中一些在六个开花阶段的表达模式。同源注释和系统发育分析表明，属于多个TF家族的某些TF，例如MADS，MYB相关，NAC和ABI3-VP1，被建议在开花过程中起重要作用。我们的数据集可用于识别优先目标TF基因以供进一步研究。

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